相关实验视频

Updated: May 11, 2025

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

诊断MicroRNA签名以支持肺高血压的分类

Niamh Errington¹, Li Zhou², Christopher J Rhodes¹

¹National Heart and Lung Institute, Imperial College London, United Kingdom (N.E., C.J.R., J.W., L.S.H., D.W., M.R.W., A.L.).

Circulation. Genomic and precision medicine

|April 18, 2025

概括

这项研究发现,虽然NT-proBNP可以识别肺高血压 (PH),但microRNA签名更好地分类PH亚组. 将NT-proBNP与miRNA签名结合起来,可以提高PH分类的准确性.

科学领域:

发现生物标志物的发现.
肺高血压研究肺高血压研究
微RNA分析的分析方法

背景情况:

肺高血压 (PH) 的分类对于治疗至关重要,但目前的生物标志物如NT-proBNP缺乏分组的特异性.
现有的PH的诊断和风险分层方法有限.
需要新的生物标志物来准确诊断和分类PH患者.

研究的目的:

识别和验证循环中的microRNA (miRNA) 签名,用于诊断和分类肺高血压 (PH).
为了比较miRNA签名与NT-proBNP的性能,用于PH检测和分类.
探索miRNA签名与临床因素的联合实用性,以改善PH亚分类.

主要方法:

从1150名PH患者和334名对照组的血清样本中分析了650个miRNA.
机器学习模型优先考虑NT-proBNP和326个miRNAs.
一般化的线性模型确定了miRNA签名来区分PH及其亚型,在独立的队列中得到验证.

主要成果:

在识别PH时,NT-proBNP显示了中度的准确性,但无法分类.
在检测PH方面,miRNA签名的性能与NT-proBNP相当,但在分类方面表现优于NT-proBNP.
结合miRNA签名,NT-proBNP,年龄和性别,在PH分类中表现优越.

结论:

NT-proBNP可以作为PH的初始查工具.
循环miRNA签名为准确的PH分类提供了一个有希望的方法.
将miRNA签名与临床数据集成,可以提高PH患者的诊断和预后能力.

关键词:

生物标志物生物标志物早期诊断早期诊断早期诊断机器学习是机器学习.米尔娜·米尔纳肺高血压是一种肺高血压.

更多相关视频

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

相关概念视频

Pulmonary Hypertension: Classification and Pathogenesis

Pulmonary Hypertension: Classification and Pathogenesis

Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...