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聚A桥接捕获探头架构使多重生物传感应用的高效DNA杂交成为可能

Jiaqi Yang¹, Lele Wang², Yanli Wen²

¹Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture; Shanghai Engineering Research Center of Aquatic-Product Process & Preservation; College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Analytical chemistry

|April 18, 2025

概括

这项研究引入了一种桥接捕获探头 (BCP) 生物传感器,可以提高电化学生物传感器的DNA杂交效率和速度. BCP战略显著提高了分子诊断和环境监测中的生物传感器性能.

科学领域:

电化学电化学电化学
纳米技术纳米技术
分子生物学分子生物学

背景情况:

传统的生物传感器由于异质的捕获探头构造而受到有限的DNA杂交动力学的影响.
这限制了电化学生物传感器的速度和实用性,特别是在现实世界样本中.
使用DNA纳米结构进行界面调节可以提高捕获探头的可访问性和活动.

研究的目的:

引入一个简化的分子调节结构,探针-多A探针 (PAP) 和捕获探针 (CP) 系统,以提高生物传感器性能.
通过与PAP的双终端杂交证明捕获探头的结构线性化,形成一个桥接的CP (BCP).
评估BCP生物传感器的效率,灵敏度和适用于各种应用的多功能性.

主要方法:

采用探针-多A-探针 (PAP) 序列的金电极表面的制造.
通过将捕获探头与PAP进行双终端混合,形成一个桥接捕获探头 (BCP).
对DNA杂交,微RNA检测和Pb2+传感的BCP生物传感器性能进行表征.

主要成果:

该BCP战略实现了捕获探头的结构线性化,提高了硬体可访问性和混合化效率.
该BCP生物传感器使得从1 fM到1 nM的快速和敏感的DNA杂交检测.
该平台展示了多功能性,成功构建了微RNA和Pb2+的生物传感器,具有快速动力学 (3分钟) 和高效率 (95.56%).

结论:

与传统方法相比,BCP生物传感器显著提高了DNA杂交动力学和效率.
BCP战略为开发高性能电化学生物传感器提供了一个多功能和强大的平台.
这种方法有望促进分子诊断和环境监测应用的发展.

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