相关实验视频

Updated: May 11, 2025

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
08:36

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

31.7K

一个快速的一步RT-qPCR测量黄热病病毒

Gisela Freitas Trindade1, Alice Gomes Fernandes2,3, Sheila Maria Barbosa de Lima1

  • 1Laboratory of Virological Technology (LATEV), Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.

Methods in molecular biology (Clifton, N.J.)
|April 18, 2025
PubMed
概括

使用定量RT-PCR (qPCR) 的新半自动方法为黄热病病毒 (YFV) 提供了快速,具体和敏感的诊断. 这项技术使用等离子体标准曲线准确量化病毒载量,以提高检测.

关键词:
自动RNA提取方式一个步骤的PCR.黄热病病毒 黄热病病毒

更多相关视频

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells07:53

Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells

Published on: January 18, 2019

8.7K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 106:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.2K

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
56.5K