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相关概念视频

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

9.7K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

12.2K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

10.4K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
10.4K

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相关实验视频

Updated: May 11, 2025

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
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Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

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免疫光检测试验

Andréa Nazaré Monteiro Rangel da Silva1, José Valter Joaquim Silva Júnior2

  • 1Institute of Biological Sciences, Virology Laboratory, Federal University of Pará, Belém, Pará, Brazil.

Methods in molecular biology (Clifton, N.J.)
|April 18, 2025
PubMed
概括

调查疑似黄热病例有助于监测和治疗. 一个新的免疫光检测 (IFA) 协议为黄热病病毒 (YFV) 诊断和研究提供了一种简单的方法.

科学领域:

  • 病毒学 病毒学
  • 免疫学 免疫学 免疫学
  • 流行病学 流行病学

背景情况:

  • 黄热病 (YF) 监测对公共卫生至关重要.
  • 早期诊断和支持性治疗对于YF患者管理至关重要.
  • 免疫光检测 (IFA) 是一种有价值的血清学工具.

研究的目的:

  • 描述一种简单的内部免疫光检测 (IFA) 协议,用于黄热病病毒 (YFV).
  • 突出IFA在YFV诊断,监测和研究中的实用性.

主要方法:

  • 开发和验证内部免疫光检测 (IFA) 协议.
  • 使用光染料标记的抗体用于YFV检测.
  • 应用IFA用于血清学诊断,病毒隔离监测和抗原检测.

主要成果:

  • 描述的IFA协议对于YFV检测是简单而有效的.
  • IFA可用于血清学诊断和监测病毒隔离.
  • IFA促进了YFV抗原和重组蛋白的检测.

结论:

  • 公司内部的IFA协议为黄热病病毒 (YFV) 研究提供了宝贵的工具.
关键词:
监测细胞转染,监测细胞转染.诊断 诊断 诊断 诊断 诊断光染料标记的抗体标记的抗体蛋白质检测检测 蛋白质检测检测血清学 血清学是一门学科.病毒隔离 病毒隔离黄热病黄热病是什么

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Last Updated: May 11, 2025

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  • 这种方法可以帮助实验室诊断和流行病学监测YF.