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Updated: May 11, 2025

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通过嵌套的RT-PCR检测对化组织中的病毒RNA

Yuli Souza1, Pedro Paulo Manso1, Marcelo Pelajo-Machado1

  • 1Laboratory of Modeling of Biological Systems (LAMES), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.

Methods in molecular biology (Clifton, N.J.)
|April 18, 2025
PubMed
概括

这项研究提出了一种有效的协议,用于检测黄热病病毒RNA在甲固定嵌 (FFPE) 组织中. 该方法克服了用于RT-PCR分析的FPE样本中常见的RNA降解问题.

关键词:
传统的PCR是传统的PCR.FFPE组织样本的组织样本嵌套的RT-PCR可以使用.帕拉芬化组织组织.黄热病基因组检测检测 黄热病基因组检测黄热病 黄热病 黄热病 RNA

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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