Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

C4 Pathway and CAM01:27

C4 Pathway and CAM

38.0K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
38.0K
Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

1.5K
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
1.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Proteolytic cleavage of G3BP1 by calpain 1 couples NMDAR activation to mTOR-dependent local translation.

EMBO reports·2026
Same author

Copper Selenides via Anion Exchange versus Direct Growth - The Role of Diorganyl Diselenides.

Inorganic chemistry·2025
Same author

Targeted genome editing of ZKSCAN3 mitigates the neurotoxicity caused by mutant HTT (huntingtin) in a Huntington disease animal model and three-dimensional cell culture of Huntington disease.

Autophagy·2025
Same author

Protein-Protein Interactions of Odorant and G Protein-Coupled Receptors: A BRET Analysis Approach.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Yellow Fluorescent Protein Quenching Assay for Analyzing Odorant Receptor Activity.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Interprofessional Training for Opioid Use Disorder Treatment: A Pilot Project of Student Outcome Analysis of Interprofessional Training.

Journal of psychosocial nursing and mental health services·2025

相关实验视频

Updated: May 3, 2026

Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals
08:30

Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals

Published on: October 31, 2011

16.0K

基于图像的cAMP传感器测试用于分析气味受体活动.

JiWoo Choi1,2, JaeHyung Koo3,4,5

  • 1Department of New Biology, DGIST , Daegu, Republic of Korea.

Methods in molecular biology (Clifton, N.J.)
|April 18, 2025
PubMed
概括

这项研究引入了一种新的基于光的测定方法,用于实时可视化气味受体 (OR) 和连接体相互作用. 这种方法有助于识别新的药物点,并了解OR功能超越鼻子.

关键词:
在GPCR中,GPCR是指GPCR.嗅觉受体是一种嗅觉受体.嗅觉受体是一种嗅觉受体.粉红色的弗拉明多火焰这是一个cAMP传感器.

更多相关视频

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
09:11

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

Published on: October 2, 2017

8.9K
Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
09:53

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Published on: April 23, 2019

7.0K

相关实验视频

Last Updated: May 3, 2026

Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals
08:30

Imaging Odor-Evoked Activities in the Mouse Olfactory Bulb using Optical Reflectance and Autofluorescence Signals

Published on: October 31, 2011

16.0K
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
09:11

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay

Published on: October 2, 2017

8.9K
Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
09:53

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Published on: April 23, 2019

7.0K

科学领域:

  • 药理学 药理学是指药理学的学科.
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 气味受体 (ORs) 是一个大型的G蛋白合受体 (GPCRs) 家庭,对于嗅觉至关重要.
  • ORs越来越多地被认为在非嗅觉组织中发挥作用,呈现出潜在的治疗点.
  • 目前对OR的功能分析受到连接物可用性和高通量查方面的挑战的限制.

研究的目的:

  • 开发一种基于成像的先进方法,用于研究OR-连接体相互作用.
  • 为了克服传统cAMP试验的局限性,用于大规模OR功能分析.
  • 为了促进ORs的脱氧化,并探索它们的非嗅觉功能.

主要方法:

  • 使用Pink Flamindo传感器,这是一个对cAMP敏感的光记者.
  • 开发了一种基于成像的实时测试方法,可在OR激活时可视化cAMP的产生.
  • 应用了对嗅觉受体的功能性特征和deorphanization的测试.

主要成果:

  • 通过光成功地展示了OR-连接体相互作用的实时可视化.
  • 展示了Pink Flamindo传感器在检测与OR活动相关的cAMP变化的实用性.
  • 验证了该试验在帮助OROR脱化方面的有效性.

结论:

  • 开发的基于Pink Flamindo的cAMP试验为研究OR提供了一个强大的平台.
  • 这种技术增强了识别OR连接体的能力,并了解它们在各种组织中的作用.
  • 该方法为药物发现提供了有价值的工具,针对GPCRs,包括气味受体.