按需调节催化DNA电路使用酸化电荷逆转
Qingqing Zhang1, Shanshan Yu1, Shizhen He1
1College of Chemistry and Molecular Sciences, Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, 430072, P.R. China.
Angewandte Chemie (International ed. in English)
|April 18, 2025
概括
研究人员开发了一种用于DNA电路的新型载体,使得微RNAs (miRNAs) 的目标体内传递和成像成为可能. 这种酶响应性系统通过减少非目标信号和保护DNA探针来提高生物传感准确性.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 纳米技术纳米技术
背景情况:
- 催化DNA电路提供高性能生物传感,但在生物体内面临交付挑战.
- 由于生物相容性和准能力,类是有希望的载体,但它们在DNA电路中的使用尚未得到充分探索.
研究的目的:
- 开发一种多功能酶响应性 (ERP),用于高效和特异性地在体内传递催化DNA电路.
- 为了使用ERP编程的DNA电路实现微RNA (miRNA) 的精确,空间控制的体内成像.
主要方法:
- 通过静电相互作用设计了一种多功能阴离子,通过电静态相互作用与阴离子DNA载荷形成稳定的纳米复合体.
- 利用基于酸化的电荷逆转机制,对酶响应的DNA探针进行细胞内释放.
- 综合活性瘤细胞向和内源酸化引导释放以提高特异性.
主要成果:
- 载体保护了DNA探针免受生物环境中的降解.
- 实现了DNA探针的特定细胞内传递和释放,减少了非目标信号泄漏.
- 通过细胞选择性传递和局部特定刺激,通过精确的,可在空间上控制的体内小RNA成像.
结论:
- 开发的ERP系统促进了DNA电路的高效加载,有针对性的传递和受控释放,用于体内应用.
- 这种方法显著提高了基于DNA电路的生物传感和疾病诊断的精度和安全性.
- 内生调节系统为体内分子成像和诊断提供了一个简单但有效的策略.
相关概念视频
Phosphorylation
49.3K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
49.3K
Covalently Linked Protein Regulators
6.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.6K
Conservative Site-specific Recombination and Phase Variation
5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
5.9K
Protein Kinases and Phosphatases
12.9K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
12.9K
Restarting Stalled Replication Forks
5.7K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.7K
Phosphodiester Linkages
97.8K
Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
97.8K


