在质体中产生的二氧化酸介导CO2和光的TOR激活
Manuel J Mallén-Ponce1, Andrea M Quintero-Moreno1, Samuel Gámez-Arcas1
1Instituto de Bioquímica Vegetal y Fotosíntesis (CSIC-Universidad de Sevilla), 41092 Sevilla, Spain.
Science advances
|April 18, 2025
概括
二氧化酸 (DHAP) 调节了雷帕素 (TOR) 激酶的点,以应对光和碳. 这项研究确定DHAP是关键的代谢物,将光合作用与Chlamydomonas中的TOR信号联系起来.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 代谢调节 代谢调节 代谢调节
背景情况:
- 拉帕素 (TOR) 激酶的标对细胞生长至关重要,并且受到光合作用生物体中光和CO2等环境线索的调节.
- 在光合作用细胞中,传输光和碳可用性到TOR的精确信号通路仍然在很大程度上没有特征.
研究的目的:
- 阐明光和二氧化碳同化调节光合作用生物中的TOR激酶活性的分子机制.
- 为了确定参与信号级联中的关键代谢物,从光线和碳线索到TOR.
主要方法:
- 在实验研究中利用了绿藻模型Chlamydomonas reinhardtii.
- 在不同的光照条件下对同步的藻类细胞进行了全面的代谢分析.
- 采用基因操纵,包括在二亚酸盐出口中存在突变缺陷,以评估TOR的代谢调节.
主要成果:
- 确定了二氧酸 (DHAP) 作为一种关键的代谢物,通过光和碳调节TOR.
- 证明DHAP水平在深色和浅色细胞之间大幅波动,与TOR活性相关.
- 表明外源二亚 (DHA),DHAP前体,在黑暗中激活TOR,TOR调节是独立于光或无机碳直接,但取决于DHAP的可用性.
结论:
- 建立了通过光和无机碳控制TOR的代谢框架,突出了DHAP的作用.
- 结论:细胞质DHAP作为一个关键的代谢信号,将光合作用状态与TOR信号通路集成在一起.
- 提供了对营养感应机制的新见解,这些机制控制了细胞在环境条件下的生长.
更多相关视频
相关概念视频
Protein Transport to the Stroma
1.8K
Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
1.8K
Protein Transport to the Outer Chloroplast Membrane
1.9K
Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
1.9K
Protein Transport to the Inner Chloroplast Membrane
2.1K
Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
2.1K
Photosystem II
69.3K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
69.3K
ATP Energy Storage and Release
8.9K
ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
One example of energy coupling using ATP involves a...
One example of energy coupling using ATP involves a...
8.9K
The Z-Scheme of Electron Transport in Photosynthesis
9.7K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
9.7K


