SOAK1和SOBIR1之间的相互酸化微调受体样蛋白 (RLP) 介导的植物免疫
Yongming Chen1, Yingying Song1, Zhipeng Tu1
1State Key Laboratory of Agricultural and Forestry Biosecurity, Ministry of Agriculture Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.
索比尔1相关蛋白激酶1 (SOAK1) 通过酸化 BIR1-1 (SOBIR1) 的抑制剂来负面调节植物免疫力. 这种酸化会损害SOBIR1的功能.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物免疫力 植物免疫力
- 类似受体的激酶信号传递.
背景情况:
- BIR1-1的抑制剂 (SOBIR1) 是一种类似受体的激酶,对植物免疫至关重要,作为多重类似受体蛋白 (RLP) 的核心受体.
- 人们对SOBIR1的稳态和活性的调节还不太了解,这代表了植物免疫信号的知识差距.
研究的目的:
- 研究SOBIR1活动的调节机制,并确定植物免疫的新型调节剂.
- 描述SOBIR1-关联蛋白激酶1 (SOAK1) 在SOBIR1-介导免疫反应中的作用.
主要方法:
- 酵母两杂交测定以确认蛋白质相互作用.
- 在体外激酶测试以确定酸化部位和作用.
- 在植物免疫信号中分析SOBIR1和SOAK1酸化突变物.
主要成果:
- SOAK1,一个类似受体的细胞质激酶V成员,负面调节RLP介导的免疫力.
- SOAK1与SOBIR1进行构成性相互作用,并在赛林-406中化它,抑制SOBIR1的转活性.
- SOBR1在-73中酸化SOAK1,抑制SOAK1的激酶活性,从而抑制SOBIR1的活性.
结论:
- 在SOBIR1和SOAK1之间有一个新的调控反循环控制植物免疫信号.
- 在-406中对SOBIR1的酸化对于调节植物免疫力至关重要.
- RLCK-V亚家族的成员在植物免疫力中发挥着重要的,以前没有表征的作用.
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