相关实验视频

Updated: May 11, 2025

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Published on: November 16, 2021

由内皮驱动的TGFβ信号支持从单细胞的肺间歇性巨细胞的发展

Wen Peng^1,2, Domien Vanneste^1,2, David Bejarano³

¹Laboratory of Immunophysiology, GIGA Institute, University of Liège, Liège, Belgium.

Science immunology

|April 18, 2025

概括

肺内皮细胞通过TGF-β1发出信号,引导从单细胞中形成间歇性巨细胞 (IMs). 这一途径对于保持肺部健康和预防与衰老相关的疾病至关重要.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
肺部病理学肺部病理学

背景情况:

肺间歇性巨细胞 (IMs) 对肺功能至关重要,但从单细胞控制它们发育的信号是未知的.
了解IM发育是解决慢性肺炎和与衰老相关的肺部疾病的关键.

研究的目的:

从单细胞识别驱动肺间歇性巨细胞 (IMs) 发育和功能规范的信号.
阐明TGF-β1信号通路在肺内的内皮细胞与巨细胞通信中的作用.

主要方法:

研究了TGF-β1在诱导小鼠骨髓衍生单细胞中的IM特征中的作用.
利用特异性TGF-β受体信号传递的骨髓体损伤来评估单细胞到IM的发展.
研究了TGF-β信号受损对肺部IM群体和小鼠衰老特征的影响.

主要成果:

肺内皮细胞衍生的TGF-β1触发了单细胞中的TGF-β受体依赖的IM特征.
在骨髓细胞或内皮细胞中破坏TGF-β受体信号传递会损害单细胞到IM的发展,减少IM数量和身份.
单细胞/IMs中TGF-β信号受损的小鼠表现出免疫调节的改变,高通胀和纤维化,这表明老化.

结论:

确定了一个关键的TGF-β信号依赖轴内皮细胞和间歇性巨细胞 (IMs) 之间的肺发育和完整性.
这一途径对于维持肺部平衡和预防与年龄相关的肺病理至关重要.
这些发现为开发针对老化和慢性炎症性肺部疾病的IM针对性干预提供了基础.

更多相关视频

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Published on: April 20, 2018

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Published on: December 21, 2014

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...