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通过RBM24介导的circ23679生物发生,通过海绵化miR-15b-5p保护心肌细胞免受亡

Jing Liu¹, Dongbo Yao¹, Fangwen Zhou¹

¹The Institute of Stem Cell and Regenerative Medicine, Women and Children's Hospital, School of Medicine, Xiamen University, Xiamen, Fujian Province 361100, PR China.

International journal of biological macromolecules

|April 18, 2025

概括

RNA结合基因蛋白24 (RBM24) 调节循环RNA (circRNA) 的产生,这对心脏健康至关重要. 依赖RBM24的circ23679作为一个microRNA海绵,防止心脏亡和心力衰竭.

科学领域:

分子生物学分子生物学
心血管生物学心血管生物学
在RNA生物学,RNA生物学.

背景情况:

循环RNAs (circRNAs) 是心脏功能和疾病的关键调节者.
RNA结合蛋白 (RBPs) 影响circRNA生物发生,但机制尚不清楚.
RNA结合动机蛋白24 (RBM24) 对于心脏功能至关重要;其缺乏会导致心力衰竭.

研究的目的:

研究RBM24在心脏中circRNA生产中的作用.
阐明RBM24介导的circRNA生物发生的机制.
确定RBM24调节的circRNAs在心脏病发生过程中的功能.

主要方法:

在野生型和Rbm24淘汰赛小鼠心脏中进行circRNA分析.
对RBM24与前mRNA结合的分析及其对外显子纳入的影响.
在心脏细胞和小鼠模型中评估circRNA-microRNA相互作用和功能后果.

主要成果:

在Rbm24淘汰赛心脏中确定了差异表达的circRNAs.
已证明,RBM24通过预mRNA结合和外子纳入直接促进circ23679的产生.
显示RBM24调节的circ23679海绵miR-15b-5p,抑制心脏亡并改善心力衰竭表型.

结论:

提出一种新型模型,其中RBPs为circRNA形成提供基质.
揭示RBM24依赖的circRNA生产作为心脏病中关键的转录后调节电路.
突出circ23679作为预防心脏亡和心力衰竭进展的保护因素.

关键词:

在RBM24中使用RBM24.灭症 (apoptosis) 是一种死亡的过程.在 circRNA 生产过程中,这是一个小RNARNA.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...