相关实验视频

Updated: May 14, 2026

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

胚胎膜上的边缘膜扩展培养:通过扩展剂对细胞表型的影响

Mehmet Gurdal¹, Kemal Baysal², Ismet Durak³

¹Dokuz Eylül University, Faculty of Medicine, Department of Medical Biochemistry, Izmir, Türkiye; Ege University, Faculty of Medicine, Ocular Surface Research Laboratory, Izmir, Türkiye; Limbustem R&D Medical Products Ltd., Ege University Technopark, Izmir, Türkiye.

Experimental eye research

|April 18, 2025

概括

通过人类羊水膜上的肢体扩展物影响细胞特征. 这一过程可能会增强肢体上皮质干细胞种群,用于治疗肢体干细胞缺乏症.

科学领域:

眼科医生眼科眼科
干细胞生物学干细胞生物学
组织工程是组织工程.

背景情况:

边缘上皮干细胞 (LESC) 对角膜健康至关重要.
淋巴干细胞缺乏症 (LSCD) 需要有效的治疗方法.
在人胎膜 (hAM) 上LESCs的体外扩张显示了LSCD治疗的前景.

研究的目的:

为了研究扩散体传递对hAM.培养的LESC表型的影响.
确定传递如何影响干细胞标记物和分化标记物.

主要方法:

在hAM上培养了子肢体扩展物,并通过两次 (通道0,1,2).
用血细胞计量细胞产量.
免疫细胞化学和RT-qPCR用于分析LESC标记物 (ABCG2,P63,CK14,CXCR4,BMI-1,维丁) 和差异化的LESC标记物 (CK3,连xin 43).

主要成果:

通过1产生了最高的细胞数量.
CK14表达保持一致;维门丁表达在通道1处达到峰值,而CK3在通道0处最高.
通过RT-qPCR检测,CK3和Connexin 43在通道0和通道2之间更高,CXCR4和BMI-1在通道1和通道0之间更高.

结论:

在hAM上传递爆炸物会改变LESC的特征.
随着通过,CK3的减少和ABCG2表达的增加表明干细胞群体的潜在丰富.
通过策略可以优化体外LESC培养物用于LSCD治疗.

关键词:

胚胎膜是一种氨基质膜.爆发式文化是一个文化.皮上皮干细胞皮上皮干细胞经过的旅行经过的旅行现象型现象型是一种现象型.

更多相关视频

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Growth of Human and Sheep Corneal Endothelial Cell Layers on Biomaterial Membranes

Published on: February 6, 2020

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

相关概念视频

Enlargement of the Plasma Membrane

Enlargement of the Plasma Membrane

Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...