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相关概念视频

RNA Editing02:23

RNA Editing

8.8K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.8K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

6.5K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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相关实验视频

Updated: May 11, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

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可编程的C-to-U编辑来追踪内源蛋白质.

Min Hao1, Tao Liu1

  • 1State Key Laboratory of Natural and Biomimetic Drugs, Chemical Biology Center, Department of Molecular and Cellular Pharmacology, Pharmaceutical Sciences, Peking University, Beijing, P.R. China.

Methods in enzymology
|April 18, 2025
PubMed
概括

研究人员开发了一种标记活细胞内蛋白质的新方法. 这种RNA编辑介导的ncAAs蛋白标记 (RENAPT) 系统允许精确跟踪内源蛋白质,有助于研究它们的功能.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学

背景情况:

  • 蛋白质标签对于了解蛋白质定位,结构和功能至关重要.
  • 在本地条件下,在活细胞中标记内源蛋白质仍然是一个重大挑战.

研究的目的:

  • 提出一种通用方法,用于活细胞内源性蛋白质的特定地点标记.
  • 通过使用最小的标签,实现各种蛋白质的实时跟踪和功能研究.

主要方法:

  • 结合可编程的cytidine到uridine (C-to-U) RNA编辑与非正规氨基酸 (ncAAs).
  • 开发RNA编辑介导的ncAAs蛋白标记 (RENAPT) 系统.
  • 将ncAAs集成到活细胞内特定部位的目标内源蛋白中.

主要成果:

  • 成功地对各种内源性蛋白质进行了特定地点的标签.
  • 证明了标记蛋白质的实时跟踪能力.
  • 验证了该系统在活细胞成像和功能研究中的适用性.

结论:

  • RENAPT提供了一个多功能平台,用于活细胞内源性蛋白质标记.
关键词:
内生蛋白质是一种内生蛋白质.非正规氨基酸的氨基酸.可编程的C到U编辑.标记蛋白质的标记

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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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Last Updated: May 11, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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A Nonsequencing Approach for the Rapid Detection of RNA Editing

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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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  • 这种技术有助于对蛋白质定位和细胞功能的深入研究.
  • 这种方法克服了原生细胞环境中现有的蛋白质标记方法的局限性.