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从昆虫细胞表达系统中净化酶活性APOBEC蛋白质

Linda Chelico1, Madison B Adolph2

  • 1Department of Biochemistry, Microbiology, and Immunology, University of Saskatchewan, Saskatoon, SK, Canada.

Methods in enzymology
|April 18, 2025
PubMed
概括

我们开发了一种baculovirus表达系统,用于净化人类APOBEC酶. 这种方法为重要的体外生化和结构研究提供了高度活性和可溶性酶.

关键词:
有助于/APOBEC酶的酶.关联性净化净化方法细菌病毒病毒的病毒.细胞因子脱氨酶酶的作用.在Sf9细胞中,

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