环境友好的合成特征和生物活动的Eruca sativa介导的氧化银纳米颗粒
Farhat Gul1, Zakir Ullah2, Javed Iqbal3
1Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.
Scientific reports
|April 18, 2025
概括
这项研究使用E. sativa提取物合成了氧化银纳米粒子 (Ag2ONPs),显示出显著的抗菌,抗氧化和抗癌特性. 这些ES-Ag2ONP显示出药物输送和生物医学应用的前景.
科学领域:
- 纳米技术和材料科学 材料科学
- 生物技术和生物医学工程 生物技术和生物医学工程
背景情况:
- 纳米结构在药物输送,诊断和治疗方面的应用方面得到了广泛的研究.
- 氧化银纳米粒子 (Ag2ONPs) 由于它们的多功能生物相互作用而引起了特别的兴趣.
研究的目的:
- 使用E. sativa (ES) 的水性提取物合成氧化银纳米粒子 (Ag2ONPs).
- 描述合成的Ag2ONP并评估它们的生物活性,包括抗氧化,抗微生物,细胞毒性和抗癌潜力.
主要方法:
- 使用E. sativa水性提取物进行Ag2ONP的生物合成.
- 使用紫外线光谱,FTIR,XRD,TEM和Zeta潜力分析进行了表征.
- 生物活性评估包括DPPH激素清除,降解功率,抗氧化能力,抗菌分析,细胞毒性测试 (Artemia salina),生物相容性测试 (VERO,HEK-293细胞系) 和抗癌分析 (Hep-2细胞系).
主要成果:
- TEM分析证实合成的Ag2ONPs的平均结晶体大小约为50nm.
- 对细菌和真菌菌株观察到显著的抗微生物活性,最大抑制区为18.5±2.36毫米对B. subtilis和16±1.7毫米对A. niger在1000μg/mL时.
- 观察到剂量依赖的生物反应,显着的IC50值用于DPPH激素清除 (62.36微克/毫升),对A. salina的细胞毒性 (LC50:12.21微克/毫升),与VERO (IC50:43.11微克/毫升) 和HEK-293 (IC50:26.56微克/毫升) 细胞系的生物相容性,以及对Hep-2细胞的抗癌活性 (IC50:9.97微克/毫升).
结论:
- 由E. sativa合成的氧化银纳米颗粒 (ES-Ag2ONPs) 具有多方面的生物特性,包括抗微生物,抗氧化剂,细胞毒性和抗癌作用.
- 这些发现凸显了ES-Ag2ONPs作为药物输送和各种生物医学应用的多功能平台的潜力.
- 建议进行进一步的深入调查和临床试验,以探索这些纳米粒子的全部翻译潜力.
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