用于基于益生菌的卫生利用的Bacillus菌株的抗体和病毒组的分析:一个多中心WGS分析

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Francesca Bini^1,2, Irene Soffritti^1,2, Maria D'Accolti^1,2

¹Section of Microbiology, Department of Environmental and Prevention Sciences, and LTTA, University of Ferrara, Ferrara, 44121, Italy.

|April 18, 2025

概括

使用细菌菌种的益生菌为基础的卫生设施是安全和基因稳定的. 这些益生菌在医院长期使用后没有获得抗菌素耐药性或毒性基因,支持它们在医疗机构的使用.

科学领域:

微生物学微生物学
遗传学遗传学是一个
环境科学环境科学

背景情况:

医疗相关感染 (HAI) 是一个日益增长的威胁,由抗菌素耐药性 (AMR) 加剧.
化学消毒剂在减少病原体的同时,可能无意中促进AMR.
基于益生菌的卫生 (PBS) 使用Bacillus spp. 提供了一种可持续的替代方案来控制病原体和AMR.

研究的目的:

为了评估PBS中使用的Bacillus益生菌的遗传稳定性.
评估在医院中长时间暴露于环境后,Bacillus分离物的潜在遗传变化.

主要方法:

全基因组测序 (WGS) 用于精确的物种识别.
分析包括单核酸多态性 (SNPs),抵抗体,病毒体和移动体概况.
在30个月的时间里,来自八家医院的环境隔离物与原始PBS产品一起被分析.

主要成果:

在原始PBS和环境样本中发现了Bacillus subtilis和Bacillus velezensis.
SNP分析显示了不同的群体,但没有获得毒性或耐药性基因.
没有检测到移动结合性等离子体,这表明水平基因转移的潜力很低.

结论:

甲基因组分析证实了PBS中使用的Bacillus益生菌的遗传稳定性.
在环境隔离物中没有观察到遗传变化或耐药性/病毒性因素的获得.
这些发现支持这些Bacillus物种在基于益生菌的卫生环境中使用的安全性.

关键词:

细菌杆菌是一种益生菌益生菌是一种益生菌.抵抗力抵抗力抵抗力卫生设施卫生设施卫生设施病毒组是病毒组.在WGS中,使用的是WGS.

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