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CSTB对羊卵细胞体外成熟的影响

Ying Chen¹, Gulimire Abudureyimu¹, Jiapeng Lin¹

¹Key Laboratory of Animal Biotechnology of XinJiang, Urumqi, China; Key laboratory of Genetics Breeding and Reproduction of Grass Feeding Livestock, Ministry of Agriculture and Rural affairs, Urumqi, China; Institute of Biotechnology, Xinjiang Academy of Animal Science, Urumqi, China.

Animal reproduction science

|April 19, 2025

概括

囊蛋白B (CSTB) 对于绵羊卵细胞成熟至关重要. 增强CSTB通过调节活性氧物种,膜潜力和自来改善卵细胞发育潜力.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
生物化学生化学

背景情况:

囊丁B (CSTB) 是一种细胞内囊素甲素抑制剂,在各种组织中起着至关重要的作用.
了解CSTB在绵羊卵巢中的功能对于改善生殖技术至关重要.

研究的目的:

调查CSTB在绵羊卵巢中的表达和局部化.
为了确定CSTB对羊卵细胞体外成熟 (IVM) 的影响.

主要方法:

从绵羊卵巢中克隆一个CSTBcDNA片段.
对CSTB氨基酸序列与其他物种相似性的分析.
在生殖组织中评估CSTB表达.
在实验室中成熟的羊卵细胞与或没有重组CSTB或CSTB倒置.
评估卵细胞成熟率,发育潜力,线粒体膜潜力,ATP水平,自标志物 (LC3A/LC3B) 和活性氧物种 (ROS).

主要成果:

克隆了绵羊的CSTBcDNA,编码了98种氨基酸,与其他物种的同类物种有很高的相似性.
在羊的卵巢和子宫中,CSTB的高度表达,局部存在于粒状细胞和卵细胞中.
再组合的CSTB添加增强了卵细胞成熟,分裂和胚胎细胞的速度.
CSTB敲击损害了卵细胞成熟和发育潜力.
CSTB调节了卵细胞中的线粒体膜潜力,ATP,自和ROS水平.

结论:

CSTB是羊卵细胞IVM的关键功能分子.
通过影响ROS,膜潜力和自,CSTB调节卵细胞的发育潜力.
这些发现有助于了解CSTB在改善羊卵细胞成熟中的作用.

关键词:

囊素B 囊素B 是一种囊素.卵细胞在体外成熟过程中卵巢卵巢卵巢羊类动物羊类动物羊类动物

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