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来自Gouqi的纳米纤维 (GqDNVs) 促进了MC3T3-E1细胞的增殖,并改善了骨折愈合

Shiyin Xu¹, Zixuan Zhang¹, Xiaolei Zhou¹

¹Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China; Department of Nutrition and Food Hygiene and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China; NHC Specialty Laboratory of Food Safety Risk Assessment and Standard Development, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.

Phytomedicine : international journal of phytotherapy and phytopharmacology

|April 19, 2025

概括

基衍生的纳米纤维 (GqDNVs) 通过增强细胞增殖和分化,促进骨折愈合. 这种传统中医成分激活PI3K/Akt/mTOR通路,改善小鼠的骨密度和愈合.

科学领域:

生物技术是生物技术.
再生医学是一种再生医学.
纳米医学是一种纳米医学.

背景情况:

Lycium barbarum L. (Gouqi) 是一种具有强化骨功能的中国传统药物.
植物外体样纳米纤维 (PELNVs) 正因其生物应用而受到关注.
来自Gouqi的纳米纤维 (GqDNVs) 提供了一个新的治疗途径.

研究的目的:

研究GqDNVs在骨折愈合中的功能和调节作用.
阐明GqDNVs作用的潜在分子机制.
评估GqDNVs在骨修复方面的治疗潜力.

主要方法:

使用糖密度梯度超离心法隔离GqDNVs.
在体外评估GqDNVs对MC3T3-E1细胞增殖和分化的影响 (CCK-8,ALP,划痕试验).
在骨折小鼠模型中的体内评估,使用Micro-CT,免疫染色和组织学.

主要成果:

GqDNVs增强了MC3T3-E1细胞活力,ALP活性和骨质蛋白表达 (OPN,ALP,RUNX2).
在体内,GqDNVs在骨折模型中增加了带厚度和骨髓密度.
在PI3K/Akt/mTOR/p70S6K/4EBP1信号通路中,GqDNVs的治疗调节了关键蛋白.

结论:

GqDNVs有效地促进骨质母细胞的增殖和分化.
在小鼠中,GqDNV显著改善骨折愈合.
GqDNVs的治疗作用与PI3K/Akt/mTOR/p70S6K/4EBP1信号通路的激活有关.

关键词:

骨折的愈合骨折的愈合基衍生的纳米纤维 (GqDNVs)这就是L. barbarum. 聚糖类是一种多糖类.骨质发生过程 (osteogenesis)植物外体类似的纳米纤维.

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