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电化学发光传感侧流免疫传感器使用光标记的银纳米颗粒,用于高度敏感和定量检测心脏特罗素I

Jun Chen¹, Xuanxu Nan¹, Li Yang²

¹School of Materials Science and Engineering, Peking University, Beijing, 100871, PR China.

|April 19, 2025

概括

一种新的电化学发光侧流免疫传感器 (ECL-LFI) 能够在非常低的水平上检测到心脏素I (cTnI). 这种敏感的传感器为急性心肌梗塞 (AMI) 的早期诊断提供了有希望的工具.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
心血管诊断心血管诊断服务

背景情况:

心脏素I (cTnI) 是诊断急性心肌梗塞 (AMI) 的关键生物标志物.
由于释放的cTnI的度非常低,早期检测AMI需要高度敏感的传感器.
现有的诊断方法可能缺乏必要的灵敏度或速度,以进行快速的临床检测.

研究的目的:

开发一种灵敏和定量电化学发光侧流免疫传感器 (ECL-LFI) 用于心脏素I (cTnI) 检测.
评估ECL-LFI用于急性心肌梗塞 (AMI) 早期诊断的性能.

主要方法:

设计了一个ECL-LFI使用灯标记的银纳米粒子 (luminol@AgNPs).
集成了印电极 (SPE) 与酸纤维素 (NC) 膜用于样本流.
利用三明治免疫测试原理在cTnI检测时产生电化学发光 (ECL) 信号.

主要成果:

该ECL-LFI显示了从5 pg/mL到100 ng/mL的广泛线性检测范围.
对于 cTnI.I. 实现了高灵敏度检测极限,低至 1.6 pg/mL.
与临床诊断测试有很好的相关性.

结论:

开发的ECL-LFI是cTnI检测的高度敏感和定量工具.
这种传感器显示出作为AMI早期检测的临床诊断工具的显著前景.
这项技术有可能应用于诊断需要生物标志物检测的其他疾病.

关键词:

心脏中位素 I 的心脏中位素 I 临床患者临床患者电化学发光的发光发生.侧流免疫测试是一种侧流免疫测试.luminol@AgNPs 这是一个很好的选择.

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