酶 (LYZ) 通过通过YTHDF2降解RIG-I转录的甲基化促进PEDV复制
Yanxia Zhang1, Qi Zhang1, Shifan Li1
1College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
International journal of biological macromolecules
|April 19, 2025
概括
酶 (LYZ) 通过抑制天生的免疫力促进猪流行性腹病毒 (PEDV) 复制. LYZ通过YTHDF2降解RIG-I转录,减少干扰素的产生,并帮助猪仔病毒扩散.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 猪流行性腹病毒 (PEDV) 在养猪业造成重大经济损失.
- 莱索酶 (LYZ),以抗菌功能而闻名,在PEDV感染中起着未经描述的作用.
- 了解宿主-病原体相互作用对于控制PEDV至关重要.
研究的目的:
- 调查Lysozyme (LYZ) 在猪流行性腹病毒 (PEDV) 复制中的作用.
- 阐明LYZ影响PEDV病变的分子机制.
- 为了确定与PEDV非结构蛋白相互作用的宿主因素.
主要方法:
- 免疫沉质谱 (IP-MS) 用于识别相互作用的蛋白质.
- 同免疫沉和激光共聚焦显微镜用于验证蛋白质相互作用.
- 基因/蛋白质表达分析,病毒复制试验和西式涂抹.
主要成果:
- 酶 (LYZ) 与PEDV非结构蛋白8 (NSP8) 相互作用.
- 在PEDV感染期间LYZ表达发生变化,转录减少和蛋白质水平增加.
- 过度表达LYZ增强了PEDV复制,而LYZ倒置则抑制了它.
- 莱兹抑制IRF3的激活和干扰素-β (IFN-β) 的生产.
- LYZ通过YTHDF2降低RIG-I转录的调节,促进RIG-I降解并抑制先天免疫力.
结论:
- 酶 (LYZ) 作为PEDV复制的积极调节剂.
- 通过向RIG-I/IFN-β途径,LYZ抑制了天生的免疫反应.
- LYZ通过YTHDF2降解RIG-I转录,为PEDV病原发生提供了一个新的机制.
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