加勒-3调节细胞感染性和由SARS-CoV2的尖端蛋白调节的炎症反应
Yuviana J Singh1, Sudhakar Singh1, Manpreet Kaur1
1Department of Biological Sciences, Indian Institute of Science Education and Research Mohali, Sector 81, SAS Nagar Knowledge City, PO, Manauli Mohali 140306, Punjab, India.
再组合的加勒-3 (Gal-3) 通过向病毒甘氨酸来抑制SARS-CoV-2感染. 加尔-3还减少了免疫细胞的炎症反应,这表明了针对COVID-19的潜在治疗策略.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 葡萄糖生物学 葡萄糖生物学
背景情况:
- SARS-CoV-2 的感染性是由其尖端 (S) 蛋白质介导的,该蛋白质具有甘氨酸部分.
- 加勒-3 (Gal-3) 是一种参与免疫反应和细胞相互作用的蛋白质.
研究的目的:
- 研究Gal-3在SARS-CoV-2感染和相关炎症中的作用.
- 探索Gal-3在中和病毒感染性方面的作用机制.
主要方法:
- 再组合Gal-3的生产.
- 使用表达SARS-CoV-2 S蛋白 (LV(CoV2-S)) 的伪型晶状病毒.
- 评估Gal-3对骨髓衍生树突细胞 (BMDCs) 病毒感染性和细胞因子产生的影响.
主要成果:
- 重组Gal-3降低了敏感细胞中的LV(CoV2-S) 感染力.
- Gal-3对LV(CoV2-S) 感染的抑制作用是由其碳水化合物识别域 (CRD) 介导的,并且可以通过α-乳糖或抗CRD抗体阻止.
- 来自Gal-3淘汰赛 (Gal-3KO) 小鼠的BMDC显示,与野生型 (WT) 小鼠相比,细胞因子反应更高.
- 外源性Gal-3的使用降低了细胞因子水平,以应对SARS-CoV-2 S蛋白衍生物.
结论:
- 通过其CRD,Gal-3可以通过与病毒甘氨酸相互作用来中和SARS-CoV-2的感染性.
- -3在调节对SARS-CoV-2的天生的免疫反应中发挥作用.
- 针对Gal-3和病毒甘氨酸之间的相互作用可能提供一种治疗策略,以减少SARS-CoV-2感染并减轻与之相关的免疫病理.
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