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Updated: May 10, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

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尼克酶忠实性驱动哺乳动物细胞的EvolvR介导多样化

Juan E Hurtado1, Adam J Schieferecke2,3, Shakked O Halperin1

  • 1Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.

Nature communications
|April 19, 2025
PubMed
概括

研究人员开发了EvolvR,这是一种使用易发生错误的DNA聚合酶的CRISPR引导系统,用于使哺乳动物细胞中的四种核酸多样化. 这使得能够发现新的遗传变异,包括耐药突变,扩展能力超出了deaminases.

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