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在猪流行性腹病毒包膜蛋白中,阿斯巴拉金-13对病原性的影响

Zhiwei Li¹, Zhiqian Ma¹, Xiaojing Zhao¹

¹State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, College of Veterinary Medicine, Lanzhou University, Lanzhou, 730000, Gansu, China.

Veterinary research

|April 19, 2025

概括

猪流行性腹病毒 (PEDV) 包膜蛋白中的特定突变显著降低了其炎症反应和致病性. 这一发现为开发减弱PEDV活疫苗提供了一个新的目标.

科学领域:

病毒学病毒学
免疫学免疫学免疫学

背景情况:

猪流行性腹病毒 (PEDV) 的发病因子尚不清楚,阻碍了疾病预防.
PEDV包裹 (E) 蛋白在诱导促炎性细胞因子中的作用及其对致病性的贡献是未知的.

研究的目的:

研究PEDV E蛋白在炎症反应中的作用.
为了确定PEDV E蛋白在诱导炎症方面至关重要的特定部位.
评估E蛋白修饰对PEDV病原性和疫苗开发的影响.

主要方法:

用局部导向的突变发生法来创建PEDV E蛋白的N13A突变.
野生型和突变E蛋白的外部表达,以评估NF-κB激活和炎症性细胞因子表达 (IL-6,IL-8,TNF-α).
重组PEDV菌株 (rPEDV和rPEDV-E_N13A) 的构建和表征,包括感染位数,斑块大小和致病性分析 in vivo.

主要成果:

位于PEDV E蛋白的13 (N13) 位置的阿斯巴拉金对于诱导炎症反应至关重要.
与野生类型相比,N13A突变E蛋白未能激活NF-κB并诱导炎症性细胞因子.
该rPEDV-E_N13A菌株显示感染位数降低,斑块较小,炎症性细胞因子表达较低,病毒RNA分泌减少,死亡率延迟,以及肠道病变较轻.

结论:

在PEDV E蛋白中的N13残留物对于诱导炎症反应至关重要,并且对病毒病原性有显著的贡献.
针对这种残留物提供了开发减弱PEDV活疫苗的潜在策略.
这项研究提供了对PEDV病原机制和潜在疫苗点的见解.

关键词:

猪流行性腹病毒病毒包裹蛋白质是一种包裹蛋白质.有助于炎症的细胞因子.反向遗传系统是一种逆向遗传系统.毒性因子是毒性的因素.

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