维原蛋白受体调解泥和斑马鱼的卵细胞发育的热适应性
Long Zhang1, Kun Wu1,2, Haoyang Li3
1School of Marine Sciences, State Key Laboratory for Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, Zhuhai, 519000, China.
Nature communications
|April 19, 2025
概括
女泥拥有热激活基因增强剂,可以保护卵细胞的发育免受高温的影响. 这种维原蛋白受体 (VtgR) 机制也出现在斑马鱼中,有助于卵子生产中的热适应性.
科学领域:
- 生殖生物学 生殖生物学
- 环境生理学环境生理学
- 基因组学就是基因组学.
背景情况:
- 气候变暖对卵性赤热虫的繁殖构成威胁.
- 保护卵细胞发育免受热应激的机制在很大程度上是未知的.
- 泥在高温下表现出可变的卵细胞发育.
研究的目的:
- 研究泥 (Scylla paramamosain) 中卵细胞发育的热应激保护机制.
- 为了确定在卵巢发育期间与耐热性有关的遗传因素.
- 探索这种机制在其他脊椎动物物种中的保护.
主要方法:
- 对泥的基因组测序和染色体水平基因组的构建.
- 鉴定和功能分析一种可热诱导的增强剂,用于维特洛根因受体 (VtgR) 基因.
- 对VtgR类基因及其在斑马鱼 (Danio rerio) 中的功能进行比较分析.
主要成果:
- 鉴定出了一种增强维泰罗基因受体 (VtgR) 基因的增强剂,这种增强剂受到高温的刺激.
- 由于内部删除,这种增强剂的损失导致了VtgR表达受损,并且在热应激下,泥的卵细胞发育异常.
- 在斑马鱼中发现了涉及Lrp13 (一种类似VtgR的蛋白质) 的类似机制,其破坏在热应激下导致卵巢退化.
结论:
- 一个VtgR介导的机制保护了粘土的维原性卵子细胞的形成,防止热应激.
- 这种机制有助于泥和斑马鱼在卵细胞发育过程中适应热量.
- 这些发现揭示了一种保存的分子策略,用于在不同物种的繁殖中应对热应激.
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