纤维化基因 CCL5 的结构和功能方面
Yi-Ting Yuan1, Tzu-Ching Guo1, Chu-Ya Wu2
1Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, 30013, Taiwan.
Scientific reports
|April 19, 2025
概括
人类CC化学基因连接体5 (hCCL5) 通过自我组装形成螺旋丝. 特定的残留物相互作用,特别是涉及R44和K45,对于这种结构及其细胞功能至关重要.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 细胞生物学 细胞生物学
背景情况:
- 人类CC化学联体5 (hCCL5) 在生理条件下表现出自我组装,但其寡合化的机制和功能尚未完全理解.
- 以前的研究表明,各种分子间相互作用调解hCCL5寡合化,使结构确定复杂化.
研究的目的:
- 阐明hCCL5寡合化和光线形成的结构基础.
- 调查hCCL5自组装的功能意义,特别是特定残留物和由此产生的光纤结构的作用.
主要方法:
- 利用hCCL5中的K25S突变来破坏特定的分子间相互作用.
- 采用传输电子显微镜 (TEM) 来可视化线形成.
- 应用X射线溶液散射和冷电子显微镜 (cryo-EM) 用于大纤维的结构确定.
- 使用核磁共振 (NMR) 光谱学确认了灯丝包装.
主要成果:
- 一个K25S突变诱导了通过TEM观察到的螺旋hCCL5丝的形成.
- 丝状聚合物成为100nM度左右的主导,在度增加时形成更高阶组件.
- 结构分析揭示了R44和K45残留物在丝包装中的关键参与.
- 确定了43TRKNR47序列对于细胞内hCCL5贩运和细胞外糖氨基酸糖结合都至关重要.
结论:
- hCCL5形成螺旋丝,由涉及R44和K45残留物的特定分子间相互作用驱动.
- 已识别的序列43TRKNR47在hCCL5的细胞贩运和细胞外相互作用中起着至关重要的作用.
- 该研究提供了对hCCL5寡合化及其功能影响的结构性见解.
关键词:
化学因子 CCL5 化学因子 CCL5化学基因分泌的分泌物发光线的发光线是非常重要的.这是NMR的NMR.斯瓦克斯 (SWAXS) 的意思低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.更多相关视频
06:56A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
Published on: March 10, 2018
13.6K
11:39Identification of Functional Protein Regions Through Chimeric Protein Construction
Published on: January 8, 2019
10.3K
相关概念视频
Mechanism of Filopodia Formation
2.2K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.2K
Structure of Cadherins
3.2K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
3.2K
Assembly of Cytoskeletal Filaments
15.0K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
15.0K
Adaptability of Cytoskeletal Filaments
3.5K
The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...
3.5K
Structural Protein Function
27.1K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
27.1K
The Structure of Intermediate Filaments
3.8K
The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm). These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate...
Intermediate...
3.8K
