拉曼光谱法用于对盐醇固体药物配方的定量和定性分析
Syeda Takmeel Zahra1,2, Noor Ul Sabah3, Muhammad Irfan Majeed4
1Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387, Krakow, Poland.
概括
拉曼光谱学有效地分析了沙布塔摩尔药物配方. 这项技术使用PCA和PLSR等化学测量工具准确地确定了固体剂型中的药物度.
科学领域:
- 制药科学 制药科学
- 分析化学 分析化学
- 频谱学是一种光谱学.
背景情况:
- 吸入药物对于喘等呼吸道疾病至关重要.
- 萨尔布塔摩尔是阻塞性肺部疾病的关键吸入药物.
- 准确的药品质量和数量评估至关重要.
研究的目的:
- 评估拉曼光谱法,以确定固体沙布塔摩尔药物配方的质量和数量.
- 评估化学计量方法 (PCA,PLSR) 在分析制药药物频谱中的有用性.
- 开发和验证一种用于预测沙布塔摩尔度的定量模型.
主要方法:
- 准备和分析了八种固体萨尔布塔摩尔药物配方.
- 使用拉曼光谱来捕获光谱数据.
- 应用主要组件分析 (PCA) 来进行光谱歧视.
- 使用部分最小平方回归 (PLSR) 进行定量分析.
主要成果:
- 拉曼光谱特征显示强度变化与配方度相关.
- 实际上,PCA有效地区分了不同的萨尔布塔摩尔配方.
- 一个PLSR模型显示出强烈的定量关系 (R2=0.998).
- 该PLSR模型准确地预测了未知配方度的低误差 (RMSECV=0.9079 mg).
结论:
- 拉曼光谱与PCA和PLSR相结合,是对固体萨尔布塔摩尔药物形式的定性和定量分析的一个强有力的工具.
- 开发的PLSR模型提供了药物配方中药物度的准确预测.
- 这种方法确保了沙布塔摩尔药物产品的质量和一致性.
更多相关视频
07:37An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
Published on: January 9, 2020
9.3K
09:24Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
Published on: August 22, 2017
8.3K
相关概念视频
01:20Raman Spectroscopy: Overview
281
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
281
01:26Raman Spectroscopy Instrumentation: Overview
256
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
256
01:24Mass Spectrum: Interpretation
1.1K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
To...
1.1K
01:21Atomic Absorption Spectroscopy: Lab
274
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
274