虫和无虫的考古组
N Dame-Teixeira1, J Lynch2, X Yu2
1Department of Dentistry, School of Health Sciences, University of Brasilia, Brasilia, Brazil.
Journal of dental research
|April 21, 2025
概括
古生物存在于口腔样本中,但它们在牙腐烂中的作用尚不清楚. 这项研究在20%的样本中发现了古生物,在虫活跃个体中,特定的基因表达不充分,这表明存在复杂的关系.
科学领域:
- 微生物学 微生物学
- 生物信息学是一种生物信息学.
- 口腔健康研究研究 口腔健康研究
背景情况:
- 由于检测和隔离困难,建立古生物和牙损伤等口腔疾病之间的联系具有挑战性.
- 古生物是具有不同代谢能力的微生物,它们在口腔中的存在需要对健康和疾病的潜在作用进行调查.
研究的目的:
- 检测和特征古生物在唾液和牙斑样本从个人和没有牙损伤使用生物信息分析的公共猎枪测序数据集.
- 调查古生物与牙腐烂和口腔细菌组的流行率和潜在相关性.
主要方法:
- 系统的文献搜索关于牙损伤的猎枪元基因组学/元转录组学研究.
- 生物信息管道使用严格的参数进行读取映射与定制的古生物基因组数据库对比.
- 流行率的元分析,多样性分析 (alpha和beta),差异丰度和差异基因表达 (使用Phyloseq和DESeq2),以及与细菌组的相关性 (斯皮尔曼).
主要成果:
- 在分析的397个样本中,63个 (20%) 呈阳性考古物,识别了Euryarchaeota,Thermoplasmatota和Nitrosphaeria.
- 甲基生物 (例如,甲生物菌 spp.) 在虫和无虫组中发现,并与特定的细菌种类正相关.
- 关键的甲基生成基因,包括甲基-辅酶M减少酶,在虫活性样本中表达不充分,而在没有虫的样本中表达不充分.
结论:
- 唾液和牙斑含有少量的古老菌,这可能对口腔健康产生潜在影响.
- 脏活跃样本中甲基基因的低表达表明,在牙损伤的背景下,古代代谢可能发挥作用,需要进一步调查.
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