在眼睛表面疾病中局部甲德的耐受性和有效性
Nathan A Seto1, Calvin W Wong2, Xiaowen Lu3
1Ophthalmology, MD Anderson Cancer Center, Houston, USA.
Cureus
|April 21, 2025
概括
局部用甲索特雷克萨特 (MTX) 在实验室中具有极小的毒性,并改善眼表面疾病患者的症状和症状. 进一步的研究正在进行中,以确认其对干眼疾病 (DED) 的治疗潜力.
科学领域:
- 眼科医生 眼科 眼科
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 炎症性干眼病 (DED) 在三级护理环境中提出了挑战.
- 对于耐火性眼表面疾病 (OSD),传统的治疗方法往往失败.
- 局部免疫调节剂越来越多地用于DED管理.
研究的目的:
- 评估局部甲状腺素 (MTX) 的体外毒性.
- 评估非标签局部MTX的治疗潜力,用于反抗性OSD.
- 确定局部MTX在患有严重DED的患者的安全性和有效性.
主要方法:
- 在体外细胞毒性测定中,使用暴露于不同MTX度的人类角膜上皮细胞和肌层细胞.
- 复发性OSD患者接受局部1mg/mLMTX治疗的患者的回顾性图表审查.
- 使用OSDI,SANDE,希尔默测试,角膜光素染色 (CFS) 和眼结膜红色 (PCR) 评估临床结果.
主要成果:
- 在24小时后,在较低的MTX度 (1-3毫克/毫升) 观察到最小的体外细胞毒性.
- 在MTX暴露96小时后,在角膜上皮细胞中观察到细胞存活率下降.
- 在接受局部MTX治疗的患者中,PCR,OSDI和CFS的显著改善 (p<0.05).
结论:
- 局部MTX在体外具有较低的细胞毒性,并且在患者中耐受性良好.
- 局部MTX在治疗反抗性DED和OSD的迹象和症状方面表现有前途.
- 需要进行更大规模的研究,以进一步验证局部MTX对OSD的疗效.
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