相关实验视频

Updated: May 10, 2025

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Published on: October 2, 2016

根据"质谱矩阵"识别Rubiae Radix et Rhizoma及其杂物

Xianrui Wang^1,2, Minghua Li^1,2, Yu Zhang^1,2

¹Institute for Control of Traditional Chinese Medicine and Ethnic Medicine National Institutes for Food and Drug Control Beijing China.

Analytical science advances

|April 21, 2025

概括

这项研究引入了一种新的"质谱 (MS) 矩阵"方法,用于检测rubiae radix et rhizoma (RRR) 中的 adulterants. 这种技术可靠地识别出即使是低水平的改,提高了RRR的质量控制.

科学领域:

药理学是指药理学,即药理学是指药理学.
分析化学分析化学
质谱测量质量谱测量

背景情况:

红叶根和根茎 (RRR) 是一种有价值的传统医学.
常见的伪造剂,如Galium elegans (GWR) 和Rubia tibetica (RTH),会损害RRR的质量和有效性.
现有法规需要加强,以有效打击RRR伪造.

研究的目的:

开发和验证一种可靠的方法来识别RRR中的伪造物.
建立一个可靠的"质谱 (MS) 矩阵"用于RRR认证.
为改善RRR改法规提供科学基础.

主要方法:

使用超高性能液态染色学与四极飞行时间联质谱学 (UHPLC-QTOF-MS/MS) 相结合.
从GWR,RTH和RRR样本中得出一个"离子表示".
从每个草药的特征离子构建了一个独特的"MS矩阵",使用化学测量验证.

主要成果:

开发的"MS矩阵"成功地将RRR与其常见的 adulterants,GWR和RTH区分开来.
该方法表现出高灵敏度,能够检测低至3%的 adulterants.
对三个市场样本的分析证实了改剂的存在,验证了该方法在现实世界中的适用性.

结论:

"MS矩阵"方法为RRR改分析提供了可靠和准确的方法.
这种技术为加强RRR质量控制和监管监督提供了一种专有和有价值的工具.
这些发现支持"MS矩阵"作为确保rubiae radix et rhizoma真实性的重要参考.

关键词:

在UHPLC-QTOF-MSE中使用.伪造法规的规定.可信度指数可信度指数质谱学质量谱矩阵.红叶根和树枝根 (rubiae radix et rhizoma) 是一种植物.

更多相关视频

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis

Published on: November 11, 2022

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

相关概念视频

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...

High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...