间歇性压力短暂发作后的机械记忆通过增加F-actin诱导YAP转位
APL bioengineering
|April 21, 2025
概括
短暂的间歇性机械应力会产生持久的细胞记忆,影响细胞行为. 这种由F-actin和YAP介导的机制记忆在消除压力后仍然存在,影响细胞功能.
科学领域:
- 细胞力学 细胞力学
- 机械生物学 机械生物学
- 生物物理学的生物物理.
背景情况:
- 了解机械力如何调节细胞功能至关重要.
- 机械记忆,即对机械扰动的持续细胞反应,是这种理解的关键.
- 之前的研究表明,机械记忆在压力后通过整合素持续约30分钟.
研究的目的:
- 测试短暂的间歇性压力事件是否通过机械传导诱导长期机械记忆.
- 研究F-actin和YAP (Yes-Associated Protein) 在压力诱导的机械记忆中的作用.
- 探索基质刚度对机械记忆的影响.
主要方法:
- 应用间歇性机械应力 (在0.3Hz时15Pa) 给中国仓鼠卵巢细胞,使用RGD涂层的磁珠与整合物结合.
- 测量了YAP和CTGF基因表达的核转位.
- 通过使用抑制剂研究了F-actin,actomyosin和微管的作用.
- 在软基质和硬基质上比较细胞反应.
主要成果:
- 多个周期的短间歇应力 (2或10分钟) 诱导了YAP核转位和CTGF表达,类似于60分钟的连续应力,但与30分钟的连续应力不同.
- 间歇性压力增加了细胞质F-actin,与YAP转位相关.
- 抑制F-actin或actomyosin,但不是微管,阻断了压力诱导的YAP转位.
- 软基板上的细胞在释放负载后显示出较大的YAP转位,而不是硬基板.
结论:
- 多次间歇性应力诱导了一个强大的细胞质机制.
- 这种机械记忆会影响细胞的生物功能,主要是通过YAP转位.
- F-actin和actomyosin网络对于传输导致YAP核定位的机械信号至关重要.
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