相关实验视频

Updated: May 10, 2025

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

基因组学协助绘制豆 (Pisum sativum L.) 早期生长的地图

Parteek Kumar¹, Saurabh Yadav², Manisha Rani¹

¹School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India.

Molecular biology reports

|April 21, 2025

概括

早期豆品种的成熟速度更快,有助于作物轮换和农民收入. 这项研究确定了一个关键的基因组区域和标记物 (PS423028253),用于培育早期成熟的豆 (Pisum sativum L.) 品种.

科学领域:

植物遗传学植物遗传学
农作物科学农作物科学
分子育种是一种分子育种.

背景情况:

花园豆 (Pisum sativum L.) 是一个温带地区的豆类,对作物轮换至关重要.
开发早期成熟品种对于提高农民收入至关重要.
了解早期生长的遗传基础是豆繁殖的关键.

研究的目的:

为了研究豆类早产的遗传学.
识别与早期成熟特征相关的基因组区域.
开发用于豆繁殖中的标记辅助选择的分子工具.

主要方法:

在F2种群上利用大规模分离分析测序 (BSA-Seq),该种群来自早期和晚期豆品种之间的交叉.
进行了QTL-seq分析,以精确确定与早产相关的主要基因组区域.
使用竞争性等位基特定PCR (KASP) 标记器验证的单核酸多态 (SNPs).

主要成果:

在豆中,早期遵循一种单一的递归遗传模式.
在染色体7上发现了一个主要的6.5 Mb的基因组区域 (被指定为PsE7),它与早产有关.
一种特定的KASP标记物PS423028253与早起特征显著相关.

结论:

已识别的基因组区域 (PsE7) 和KASP标记物 (PS423028253) 对于在豆繁殖中进行标记器辅助选择非常有价值.
这些发现将促进早期成熟的豆品种的发展.
这项研究为未来研究控制豆早产的候选基因提供了基础.

关键词:

这就是BSA-seqq.早期的时间早期的时间继承权继承权继承权卡斯普 (KASP) 公司标记器辅助的选择国家统一计划 (SNP) 是一个国家统一计划.

更多相关视频

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

相关概念视频

Monohybrid Crosses

Monohybrid Crosses

Dihybrid Crosses

Dihybrid Crosses

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...