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Updated: May 16, 2025

Counting Proteins in Single Cells with Addressable Droplet Microarrays
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Counting Proteins in Single Cells with Addressable Droplet Microarrays

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基于单细胞成像数据表达水平分布的功能性蛋白质生物标志物

Misung Yi1, Tingting Zhan2, Hallgeir Rui3

  • 1Department of Statistics & Data Science, College of Software and Convergence, Dankook University, Suji-gu, Gyeonggi-do 16890, Korea.

Bioinformatics (Oxford, England)
|April 21, 2025
PubMed
概括

我们开发了一个新的量子指数 (QI) 生物标志物框架,使用单细胞蛋白质表达分布来预测癌症的结果. 非线性QI生物标志物在乳腺癌数据集中表现优异,为癌症预后提供了更有效的方法.

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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