Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Combinatorial Gene Control02:33

Combinatorial Gene Control

8.2K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.2K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.1K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.1K
General Transcription Factors01:30

General Transcription Factors

5.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.1K
Reporter Genes02:11

Reporter Genes

11.0K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.0K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.2K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.2K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

845
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
845

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Evaluation of analysis modes for RNA coexpression in single-cell and bulk tissue.

bioRxiv : the preprint server for biology·2026
Same author

Application of large language models to the annotation of cell lines and mouse strains in genomics data.

bioRxiv : the preprint server for biology·2026
Same author

Thiorphan reprograms neurons to promote functional recovery after spinal cord injury.

Nature·2025
Same author

Global partnerships in rare disease research.

Disease models & mechanisms·2025
Same author

Revealing function-altering MECP2 mutations in individuals with autism spectrum disorder using yeast and Drosophila.

Genetics·2025
Same author

Identifying Reproducible Transcription Regulator Coexpression Patterns with Single Cell Transcriptomics.

bioRxiv : the preprint server for biology·2024

相关实验视频

Updated: May 10, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.6K

识别可复制的转录调节器同表达模式与单细胞转录组学.

Alexander Morin1,2,3, Ching Pan Chu1,2,3, Paul Pavlidis1,2

  • 1Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada.

PLoS computational biology
|April 21, 2025
PubMed
概括
此摘要是机器生成的。

这项研究在数百万个细胞中绘制基因协同表达网络,以确定人类和小鼠转录调节器 (TRs) 的基因伙伴. 这些发现揭示了可复制的调节模式,有助于理解基因转录动态.

更多相关视频

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

29.7K
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.4K

相关实验视频

Last Updated: May 10, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

6.6K
Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

29.7K
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

13.4K

科学领域:

  • 基因组学就是基因组学.
  • 计算生物学 计算生物学
  • 分子生物学分子生物学

背景情况:

  • 单细胞转录组学能够对基因转录调节进行详细的分析.
  • 了解转录调节器 (TR) 网络对于破译细胞过程至关重要.

研究的目的:

  • 使用大规模单细胞RNA-seq数据,与人类和小鼠的转录调节器 (TRs) 协调识别基因合作伙伴.
  • 评估TR共同表达特征在各种生物环境和物种中的一致性.

主要方法:

  • 组装了120个人类和103个小鼠单细胞RNA-seq数据集 (>2800万个细胞).
  • 为人类和小鼠TRs构建的单细胞同表达网络.
  • 在细胞类型和物种中优先重复可重复的共同表达模式.

主要成果:

  • 为每个TR生成单细胞同表达排名,与文献策划的目标进行验证.
  • 在识别TR目标方面实现了与ChIP-seq数据相比较的性能.
  • 集成的协同表达和ChIP-seq数据,以确定强大的候选监管相互作用.

结论:

  • 这项研究为了解转录监管网络提供了全面的资源.
  • 确定了跨物种和细胞类型的可复制的基因协同表达模式.
  • 强调综合数据对于发现新型监管相互作用的有用性,以ASCL1.1为例.