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醇和相关代谢特征的呼吸分析:使用二次电喷离子化高分辨率质谱的试点研究

Jiafa Zeng1,2,3, Nikola Stankovic4, Kapil Dev Singh1,2

  • 1Department of Biomedical Engineering, University of Basel, Basel, Switzerland.

Anesthesiology
|April 21, 2025
PubMed
概括

呼吸分析提供了实时的洞察力,了解在手术期间的propofol麻醉和代谢压力. 这种药物代谢学方法显示了个性化麻醉护理和监测患者反应的前景.

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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