鱼类疫苗可以促进大西洋鱼对病原体的血细胞转录重塑
Yeny Leal1, Valentina Valenzuela-Muñoz1, Cristian Gallardo-Escárate1
1Interdisciplinary Center for Aquaculture Research (INCAR), Universidad de Concepción, P.O. Box 160-C, Concepción, 4030000, Chile.
Fish & shellfish immunology
|April 21, 2025
概括
大西洋鱼血细胞转录显示了免疫对疫苗接种和感染的反应. IPath®疫苗引起了比商业疫苗更强烈的反应,调节铁代谢并激活缺氧通路.
科学领域:
- 水产养殖免疫学 水产养殖免疫学
- 鱼类健康管理 鱼类健康管理
- 分子诊断学 分子诊断学
背景情况:
- 智利鱼养殖面临的疾病挑战包括卡利吉多सिस和瑞克特鱼性血病 (SRS).
- 疫苗接种是减少水产养殖中抗生素使用的可持续策略.
- 血细胞转录学提供了一种非致命的方法来研究鱼类的免疫反应.
研究的目的:
- 评估大西洋鱼血细胞对接种疫苗和随后与海和Piscirickettsia salmonis的挑战的转录基因反应.
- 为了比较由重组IPath®疫苗引起的免疫反应与两个商业疫苗.
- 确定通过疫苗接种和感染调节的关键免疫通路.
主要方法:
- 在四个组进行疫苗接种试验:IPath®,BlueGuard®,Alpha Ject LiVac® SRS和未接种疫苗的对照组.
- 对大西洋鱼血细胞转录组的分析.
- 基因表达分析,以评估免疫反应的动态.
主要成果:
- 与商业疫苗相比,IPath®疫苗组表现出更高的转录组反应.
- 所有接种疫苗的群体都调节了参与铁平衡和代谢的基因.
- 在IPath®组中,HIF-1信号通路和铁亡被激活,表明在低氧反应和细胞死亡中的作用.
结论:
- 大西洋鱼的血细胞是有效的监测免疫反应的疫苗和病原体.
- IPath®疫苗显示出强大的免疫刺激特征.
- 了解血液细胞转录组学,可以了解鱼类免疫力,而无需致命的采样.
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