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无侧环决定性3D纳米镜,具有λ/33轴分辨率

Binxiong Pan¹, Baoju Wang¹, Yue Ni¹

¹Centre for Optical and Electromagnetic Research & Guangdong Engineering Research Centre of Optoelectronic Intelligent Information Perception, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou, China.

Light, science & applications

|April 21, 2025

概括

我们开发了一种新的3D超高分辨率显微镜技术,称为UNEx-4Pi. 这种方法实现了高轴分辨率,并消除了侧叶,使得精确的纳米尺度成像和其他先进的应用.

科学领域:

光学和光子学在光学和光子学.
生物物理学的生物物理.
纳米技术纳米技术

背景情况:

确定性3D超分辨率显微镜经常与轴向分辨率作斗争,通常显示较长的焦点.
像isoSTED这样的现有方法提供了改进的轴分辨率,但引入了光学复杂性和侧叶.
高级非线性光学效应可以提高分辨率并抑制侧叶.

研究的目的:

开发一种易于使用的,无侧环的确定性3D纳米技术,具有高轴分辨率.
为了实现先进的应用,在有限的体积中实现精确的光物质相互作用.

主要方法:

开发了UNEx-4Pi策略,将使用光子雪崩纳米粒子的超高非线性激发 (UNEx) 与基于镜子的双焦向量场调制 (4Pi) 结合起来.
利用镜像辅助的单一目标双焦自我干扰策略来实现系统的简单性和稳定性.
通过理论研究研究了光学非线性对焦点特征和侧叶抑制的影响.

主要成果:

理论研究证实了更尖的焦点和压抑的侧叶,增加了光学非线性.
在实验中,使用单个低功率连续波束,实现了高达 λ/33 (26 nm) 的轴分辨率的无侧叶焦点.
证明了BSC-1细胞核外的生物成像,轴分辨率为32nm.

结论:

UNEx-4Pi方法为3D超分辨率成像提供了一个简单,稳健和有效的确定性策略,具有特殊的轴分辨率.
这种技术克服了现有方法的局限性,通过实现无侧叶片成像和高轴束限度.
UNEx-4Pi具有在超高分辨率传感,成像,石版和数据存储方面的技术进步的巨大潜力.

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