在致病性大肠杆菌Escherichia coli O157:H7中纳米塑料介导的生理和基因组反应
Jayashree Nath1, Goutam Banerjee1, Jayita De1
1Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Journal of nanobiotechnology
|April 21, 2025
概括
带正电荷的纳米塑料 (NP) 抑制了致病性大肠杆菌的生长,改变了生物膜的形成. 虽然不影响生存,但NP显著影响病原体的毒性和应激反应,突出环境风险.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 微塑料 (MP) 和纳米塑料 (NP) 是无处不在的环境污染物.
- MP/NP对微生物,特别是人类病原体的影响尚不清楚.
- 病原细菌与MP/NP相互作用,可能改变其生理学和行为.
研究的目的:
- 调查表面功能化,带电差的聚钢 (PS) NP对人类病原体大肠杆菌O157:H7.7.的影响.
- 评估充电NP对细菌生长,活力,毒性,应激反应和生物膜形成的影响.
主要方法:
- 对大肠杆菌O157:H7对正,负和未充电的PS NPs的实验暴露.
- 细菌生长,活力和生物膜形成的分析.
- 转录和基因表达分析以确定代谢途径,应激反应和毒性基因的变化.
主要成果:
- 带正电荷的NP对浮游生物大肠杆菌生长表现出细菌静止作用,并影响了细胞活力和生物膜启动.
- NP改变了全球基因表达,包括与压力反应和毒性相关的途径.
- 在NP暴露的生物膜中观察到Shiga类毒素 (stx1a),定数感应和生物膜启动基因的升级.
- 病原体生存没有受到显著影响,但生长,生物膜发育和毒性特征受到影响.
结论:
- 充电的NP会影响致病性大肠杆菌的生理学,包括生长,活力和生物膜形成.
- 核瘤显著影响细菌的毒性特征和应激反应,特别是滋果类毒素的产生.
- 对NP的环境暴露通过改变病原体行为和增强毒性而带来风险,尽管没有显著影响生存.
更多相关视频
06:20Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
Published on: April 9, 2018
9.4K
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
2.1K
相关概念视频
01:23Stringent Response in E. coli
528
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
528
01:28Microbial Bioremediation of Plastics
131
Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...
131
01:18Bacterial Gastroenteritis
88
Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid...
88