在协同光热瘤治疗中,NIR触发的siRNA释放和溶酶体逃逸
Myagmarsuren Baldan1,2,3, Shuang Zhang1,2,3, Qi Sun1,2,3
1School of Pharmaceutical Sciences, Capital Medical University, Beijing, People's Republic of China.
International journal of nanomedicine
|April 22, 2025
概括
这项研究开发了载有绿色氨酸 (ICG) 和向热冲击蛋白70 (HSP70) 的siRNA的基于白蛋白的纳米粒子,以克服瘤耐药性. 这些纳米颗粒有效地使HSP70沉默,并增强光热疗法,显著抑制瘤生长,毒性最小.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 癌症治疗 癌症治疗
背景情况:
- 光热疗法 (PTT) 对瘤切除有希望,但面临着由热冲击蛋白70 (HSP70) 介导的抵抗.
- 将PTT与HSP70的基因沉默相结合,可以提高治疗疗效.
- 基于白蛋白的纳米颗粒 (NP) 提供了一个平台,用于联合传递诸如绿色氨酸 (ICG) 和siRNA等光热剂.
研究的目的:
- 开发和评估HSA@ICG/siRNANP作为一种生物相容的系统,用于联合光热和基因沉默疗法.
- 调查这些NP在克服瘤耐药性和增强癌症治疗中的有效性.
- 评估开发的纳米治疗药物的体外和体内性能.
主要方法:
- 基于白蛋白的纳米粒子 (HSA@ICG/siRNA NPs) 对于尺寸,电荷和光转换的合成和表征.
- 细胞吸收,溶酶体逃逸,基因沉默 (mRNA/蛋白质减少) 和4T1瘤细胞中细胞毒性的体外评估.
- 在体内评估光热效应,生物分布,瘤抑制率和近红外 (NIR) 光照射后的生物安全性.
主要成果:
- HSA@ICG/siRNANP已成功合成,具有统一的尺寸和有效的光热特性.
- 试验室研究表明,siRNA的有效传递,细胞质释放的增强以及HSP70mRNA (82.8%) 和蛋白质 (65%) 的显著减少.
- 在体内,NIR照射诱导轻度高温 (42°C),导致瘤抑制 (71.26% ± 7.92%) 与单独PTT (50.00% ± 9.16%) 相比,具有最小的目标外毒性.
结论:
- HSA@ICG/siRNANP作为双重功能剂,将向基因沉默与光热疗法结合起来.
- 这种综合方法有效地克服了瘤耐药性,并显示出显著的治疗潜力.
- 开发的纳米载体代表了多模式癌症治疗策略的有希望的进步.
相关概念视频
Tumor Immunotherapy
398
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
398
Targeted Cancer Therapies
7.4K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.4K


