双核 (III) 复合物与1,3-二胺-N,N'-二甲酸盐:结构特征,抗菌活性和DNA/BSA相互作用
Bojana V Pantović1, Darko P Ašanin2, Žiko Milanović2
1Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, Kragujevac 34000, Serbia.
Bioinorganic chemistry and applications
|April 22, 2025
概括
这项研究合成了一种双核 (III) 复合物,uns-cis-[Ga(1,3-pdda) ((μ-OH) ]2·2H2O,显示出针对Pseudomonas aeruginosa的选择性抗菌活性. 该复合体还调节了定数感应,并与DNA和BSA相互作用.
科学领域:
- 协调化学 协调化学
- 无机合成 无机合成
- 抗菌剂 抗菌剂 抗菌剂
背景情况:
- (III) 复合物正在探索治疗应用.
- 了解连接体对复杂结构和功能的影响至关重要.
- 准细菌通信系统,如定数感应,提供了新的抗菌策略.
研究的目的:
- 为了合成和表征一种新的双核(III) 复合物,使用一个四体1,3-propanediamine-N,N'-diacetate (1,3-pdda2-) 配体.
- 评估合成复合体对各种微生物物种的抗菌潜力,重点是Pseudomonas aeruginosa.
- 研究该综合体与生物分子的相互作用,如小牛胸腺DNA (ct-DNA) 和牛血清白蛋白 (BSA).
主要方法:
- 合成双核 (III) 复合物uns-cis-[Ga(1,3-pdda) ((μ-OH) ]2·2H2O (1) 的合成.
- 使用IR,NMR光谱和单晶X射线衍射进行了表征.
- 抗微生物活性测试,皮约素产量减少测试,定数感应调制研究,DNA/BSA结合测试 (光谱度,分子对接) 和DFT模拟.
主要成果:
- 双核 (III) 复合物 (1) 已成功合成和结构特征,揭示了每个Ga (III) 离子周围的八面体几何形状.
- 综合体1对Pseudomonas aeruginosa PAO1表现出选择性抗菌活性,减少了40-43%的皮奥素产量,并调节了其定数感应系统.
- 综合体1在BSA上表现出强烈的结合到Site I,并且对ct-DNA表现出较小的沟结合亲和力.
结论:
- 合成的双核 (III) 复合物具有对 Pseudomonas aeruginosa 有望的抗菌特性.
- 复合体干扰细菌的定数感应的能力突出显示了其作为一种新型抗感染剂的潜力.
- 与BSA和ct-DNA的相互作用表明生物运输的潜在机制以及与DNA的一般安全相互作用概况.
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