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Updated: May 8, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

微流体平台用于快速多重化生物样本处理,用于分子询问.

Seth R T Zima^1,2,3, Rithvik V Turaga^1,2,3, Sofia C Yeates-Delahoz^1,2,3

¹Department of Dermatology, School of Medicine and Public Health, University of Wisconsin, 1 S Park Street, Madison, WI, USA. ayusodomingu@wisc.edu.

|April 22, 2025

概括

这项研究引入了一种用于多重化生化分析的新型微流体装置,简化了细胞研究. 具有毛细血管的创新井板设计允许对像干扰素马 (IFN-γ) 这样的免疫标记物进行一致的,高通量查.

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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

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Published on: November 23, 2015

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

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Last Updated: May 8, 2025

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

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Published on: March 13, 2017

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

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Published on: November 23, 2015

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

科学领域:

生物技术是生物技术.
免疫学免疫学免疫学
微流体学微流体学

背景情况:

在细胞制造和免疫研究中选分析物通常需要昂贵,劳动密集型的方法,如流细胞计.
现有的微流体设备往往需要专门的设备和专业知识来制造和使用.
需要在细胞生物学和免疫学中提供可访问,高通量分析工具.

研究的目的:

设计和验证用于多重化生化分析剂分析的微流体装置.
简化和提高细胞制造和免疫细胞研究中的测试效率.
为了证明该设备测量关键免疫标记物的能力.

主要方法:

开发了一种微流体装置,集成到标准井板格式中.
整合了毛细管,以精确地限制液体和冷稳定性.
生物发光ATP测定T细胞活力的性能.
进行生物发光免疫测试以测量干扰素- (IFN-γ) 分泌.

主要成果:

微流体装置促进了同时向多个井输送液体,减少了管道输送的步骤.
毛细管显示出对结解周期的稳定性,使试剂预加载成为可能.
在ATP可行性测试中实现了对发光信号的一致捕获.
该装置成功地区分了原始和激活的CD4+T细胞之间的IFN-γ分泌水平.

结论:

开发的微流体井板装置为高通量生物化学分析提供了简化,一致和潜在的成本有效的解决方案.
这项技术在推进免疫细胞研究和监测相关免疫标记物方面具有重大潜力.
该设备的设计支持试剂储存,并使多重分析成为可能,提高了测试效率.