在Sprague-Dawley大鼠单剂量口服后,对载体对选定的生物参数的影响的比较分析
Tae-Woo Kim1,2, Tae-Kyung Kim1,2, Hye-Joon Park2
1Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
International journal of toxicology
|April 22, 2025
概括
动物研究中的载体物质可能会影响结果. 在斯普拉格-道利大鼠中,二甲基硫氧化物 (DMSO) 显著改变了红细胞计数和器官重量,这强调了在非临床研究中需要仔细选择载体的必要性.
科学领域:
- 毒理学 毒理学 毒理学
- 药理学 药理学是指药理学的学科.
- 动物模型 动物模型
背景情况:
- 车辆对于在非临床研究中给予物质至关重要.
- 车辆管理可以影响动物生理学和研究结果.
- 关于普通车辆对生物的影响的研究不足.
研究的目的:
- 评估常用车辆对斯普拉格-道利大鼠的生物学影响.
- 为了建立由车辆影响的生物参数的参考范围.
- 为非临床毒性研究提供历史控制数据的信息.
主要方法:
- 1,528只斯普拉格 - 达利大鼠被口服用5毫升/公斤/天的载体 (蒸水,玉米油,DMSO,PEG 400,CMC,MC) 给药.
- 评估的参数包括体重,食物/水消耗,功能观察电池,临床病理和器官重量.
- 剂量方案模仿了毒性测试中使用的剂量方案.
主要成果:
- 甲基二硫氧化物 (DMSO) 显著改变了红细胞计数和器官重量.
- 其他测试的车辆只引起了轻微的生理变化.
- 建立了关键生物参数的参考范围.
结论:
- 在非临床研究中,选择车辆至关重要,以尽量减少生物影响.
- 与其他车辆相比,DMSO表现出显著的生理效应.
- 调查结果有助于选择合适的车辆和解释毒性研究数据.
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