微流体动脉在芯片上的模型,具有单向重力驱动的流量,用于高通量应用
H Ehlers1,2, T Olivier1, S J Trietsch1
1Mimetas B.V., Oegstgeest, The Netherlands. l.vandenbroek@mimetas.com.
Lab on a chip
|April 22, 2025
概括
开发新的心血管疾病 (CVD) 疗法需要更好的体外模型. 这项研究提出了一个新的微流体平台,可以准确地模仿健康和患病的动脉,改善心血管疾病治疗的临床前研究.
科学领域:
- 生物医学工程 生物医学工程
- 心血管研究研究心血管研究
- 微流体学 微流体学
背景情况:
- 心血管疾病 (CVD) 仍然是全球主要的死亡原因.
- 现有的心血管疾病临床前模型缺乏预测性临床疗效.
- 需要先进的体外模型来更好地复制人类心血管生理学.
研究的目的:
- 开发和验证一个新的微流体平台来建模人类冠状动脉.
- 研究不同流量条件对血管细胞行为和功能的影响.
- 建立一个更生理相关的体外系统,用于心血管研究和药物查.
主要方法:
- 使用OrganoPlate® 2-lane-48 UF微流体装置与人类冠状动脉内皮细胞 (HCAECs) 和人类冠状动脉光滑肌细胞 (HCASMCs) 的单培养或共同培养.
- 通过间隔摇摆和毛细血管力应用单向和双向流体流动条件.
- 评估了内皮细胞对齐,纤维内素沉积,光滑肌肉细胞表型,ICAM-1染色和脂质沉积.
- 使用TNFα和IL-1β诱导的血管炎症.
主要成果:
- 单向流动促进了内皮细胞的对齐,并降低了纤维素,模仿健康的动脉.
- 双向流动诱导了早期内皮功能障碍的特征,包括收缩形态,纤维素蛋白增加,ICAM-1染色和脂质沉积.
- 该平台成功地复制了健康和患病动脉的关键特征,并允许诱导血管炎症.
结论:
- OrganoPlate® 2-lane-48 UF是一个高通量,流量控制的平台,用于创建生理学上相关的体外动脉模型.
- 这种模型准确地复制了健康和患病动脉的特征,为改善心血管药物查提供了潜力.
- 该平台与实验室自动化的兼容性使其成为推动心血管疾病治疗开发的宝贵工具.
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