Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Loss of O-antigen due to <i>wbbL</i> mutations is common and associated with increased mortality in <i>Escherichia coli</i> bloodstream infections.

Science advances·2026

Same author

Power and sample-size estimation in human microbiome research.

Med (New York, N.Y.)·2026

Same author

Species and strain diversity in Staphylococcus drive divergent host responses in human skin.

bioRxiv : the preprint server for biology·2026

Same author

Deep single-cell decoding of human pancreatic islets reveals T2D β-cell gene expression defects.

The EMBO journal·2026

Same author

Distinct biochemical phenotypes of HIV exposed infants driven by antiviral medication.

medRxiv : the preprint server for health sciences·2026

Same author

PD-L1 is an intrinsic switch for natural killer cell-mediated, TRAIL-dependent antiviral function.

Cell reports·2026

Same journal

Salinity-driven microbial adaptation of hydrocarbon-degrading communities in coastal sediments.

mSphere·2026

Same journal

Tools for genetic manipulation of the endemic fungal pathogen <i>Emergomyces africanus</i> and application of a fluorescent reporter strain in infection models.

mSphere·2026

Same journal

Cysteine-S-nitrosylation inhibits ROP5-mediated immune evasion in <i>Toxoplasma gondii</i>.

mSphere·2026

Same journal

Inheritance of four-membrane-bound structures in the "apicoplast-minus" <i>Plasmodium falciparum</i>.

mSphere·2026

Same journal

mSphere of Influence: The bacterial growth law-revisiting the old to discover the new.

mSphere·2026

Same journal

Preclinical characterization of immune responses induced by a candidate gonococcal native outer membrane vesicle vaccine.

mSphere·2026

查看所有相关文章

Search research articles

相关实验视频

Updated: May 10, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

TEAL-Seq:针对性的表达式分析测序.

Georgia Doing¹, Priya Shanbhag¹, Isaac Bell¹

¹The Jackson Laboratory for Genomic Medicine Farmington, Farmington, Connecticut, USA.

|April 22, 2025

概括

此摘要是机器生成的。

我们开发了针对性表达分析测序 (TEAL-seq),用于微生物群体中敏感的,特定物种的基因表达分析. 这种方法克服了低生物质和宿主RNA等挑战,为微生物组研究提供了具有成本效益和适应性的解决方案.

关键词:

转基因组学是指转基因组学.皮肤的微生物组有针对性的测序.

更多相关视频

3' End Sequencing Library Preparation with A-seq2

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

相关实验视频

Last Updated: May 10, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

3' End Sequencing Library Preparation with A-seq2

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

科学领域:

微生物学微生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

甲基因组测序对微生物群落有特征,但由于丰度变化和生物量低,难以量化基因表达.
主体RNA和低微生物生物量使复杂环境中的微生物活动的准确评估变得复杂.

研究的目的:

开发一种敏感和特定的方法来分析复杂社区内的向微生物物种的基因表达.
解决现有方法在检测微生物群中的特定物种活动方面的局限性.

主要方法:

开发了"定向表达分析测序" (TEAL-seq),使用高度多重化的定制探针池进行特定物种的基因表达分析.
应用TEAL-seq与分子反向探针和线性预放大步骤到实验室培养物和人类鼻.
向了大约1,700个黄金葡萄球菌和S. epidermidis*的核心和辅助基因.

主要成果:

TEAL-seq表现出高特异性 (>90%读取目标) 即使具有复杂的背景DNA/RNA.
来自TEAL-seq的推断表达水平与散装RNA-seq.q.有很好的相关性.
从1 ng的总RNA中进行分析是成功的,在低生物质样本中证明了有效性.

结论:

TEAL-seq为分析任何社区微生物基因表达提供了具有成本效益,灵敏和适应性的策略.
该方法可以在较大的动态范围中进行检测,而不是大量的元转录形状分析.
TEAL-seq促进了在各种自然环境中研究微生物活动和相互作用的研究.