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自动全基因组测序平台用于临床微生物学实验室的细菌菌株类型

Rachel L Siddall¹, Jordan C Starkey¹, Robin Patel^1,2

¹Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.

Journal of clinical microbiology

|April 22, 2025

概括

一个新的全基因组自动测序 (WGS) 平台通过减少周转时间和成本,显著改善了细菌菌株类型. 这种自动化方法几乎与手动WGS的准确性相匹配,提高了疫情调查的实验室效率.

科学领域:

临床微生物学临床微生物学
基因组学就是基因组学.
传染病流行病学传染病流行病学

背景情况:

隔壁疫情对患者的安全构成风险,并增加医疗保健成本.
全基因组测序 (WGS) 对于调查疫情至关重要,但传统方法耗时.
手动DNA提取和库准备是当前细菌WGS工作流程中的瓶.

研究的目的:

评估一个自动化图书馆准备和细菌WGS的测序平台.
为了比较自动化Clear Dx WGS平台与手动方法的性能.
评估自动化对细菌WGS的周转时间,劳动力和成本的影响.

主要方法:

将一个完全自动化的WGS平台 (Clear Dx) 与一个手动的图书馆准备和测序方法进行比较.
通过两种方法分析了来自18个物种的226个细菌分离物.
使用SeqSphere+软件进行序列数据分析和结果比较.

主要成果:

几乎获得了同等的结果,自动化和手动方法之间的99%一致的分离分组.
自动化工作流减少了16-19小时的交付时间,并消除了3小时的手工劳动.
据估计,自动化方法节省了34%至57%的成本.

结论:

自动化Clear Dx WGS平台提供与手动方法相比,准确可靠的细菌菌株类型.
自动化通过减少动手时间,周转时间和整体成本,显著提高效率.
将自动化集成到细菌WGS工作流程中,为疫情调查和实验室操作提供了巨大的优势.

关键词:

核心基因组多部位序列的类型化.实验室自动化实验室自动化整个基因组的测序.

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