相关实验视频

Updated: May 10, 2025

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Published on: August 29, 2012

混合淋巴细胞反应条件的MSC衍生的细胞外囊通过miR-638介导的免疫调节提高了移植的存活率

Yue Ding¹, Jiyuan Wang¹, Xueyang Zheng¹

¹Department of Organ Transplantation, Shanghai Changzheng Hospital (Second Affiliated Hospital of Naval Medical University), 200003 Shanghai, People's Republic of China.

Stem cells translational medicine

|April 22, 2025

概括

用混合淋巴细胞反应条件介质 (MLR-CdM) 进行介质介质介质干细胞 (MSCs) 增强其细胞外囊泡 (EVs),以改善免疫抑制. 采用MLR-CdM的MSC-EV通过miR-638/Fosb通路促进移植的生存和免疫耐受性.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
再生医学是一种再生医学.

背景情况:

介酶干细胞 (MSCs) 需要进行原始化,以实现最佳的免疫抑制.
混合淋巴细胞反应条件介质 (MLR-CdM) 增强了MSC的免疫抑制特性.
这项研究将MLR-CdM原料的MSC细胞外囊泡 (MSC-EVMLR) 与IFN-γ原料的 (MSC-EVIFN) 进行比较.

研究的目的:

与MSC-EVIFN相比,评估MSC-EVMLR的免疫调节潜力.
确定关键的微RNA (miRNA) 和参与MSC-EVMLR功能中的机制.
在移植模型中评估MSC-EVMLR的疗效.

主要方法:

在体外评估淋巴细胞增殖和细胞因子表达.
微RNA阵列分析和剂量反应实验以确定效应分子.
鼠类异种异种心脏移植模型,以评估体内疗效.

主要成果:

与MSC-EVIFN相比,MSC-EVMLR表现出更好的淋巴细胞增殖抑制,并促进了抗炎性特征.
在MSC-EVMLR中,miR-638被确定为一个关键的效应分子.
在体内,MSC-EVMLR显著延长了移植存活时间,降低了IL6和IFN-γ,并增加了调节性T细胞 (Tregs),影响取决于miR-638和miR-638/Fosb轴.

结论:

通过MLR-CdM预先调节,可以增强MSC-EV免疫调节能力,超过IFN-γ.
这项研究阐明了基于MSC-EV的移植疗法的机制,突出了miR-638/Fosb途径.
在移植环境中,MSC-EVMLR显示出增强免疫耐受性的前景.

关键词:

这就是Treg Treg.植入物生存率植入物生存率介酶干细胞衍生的细胞外囊泡.在 miR-63838 里面.混合的淋巴细胞反应条件介质.

更多相关视频

Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease

Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease

Published on: March 17, 2020

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

Published on: August 12, 2017

相关概念视频

Cell-mediated Immune Responses

Cell-mediated Immune Responses

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...