Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

CRISPR01:59

CRISPR

48.6K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
48.6K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.4K
Homologous Recombination02:31

Homologous Recombination

49.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
49.9K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

9.6K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The dual role of ATF4 in neurons: from stress adaptation to therapeutic intervention.

Frontiers in molecular neuroscience·2026
Same author

A Survey on Human-Centric Voice-Face Multimodal Learning.

IEEE transactions on neural networks and learning systems·2026
Same author

DOGT: Double-Order Graph Transformers With Adaptive Node-Group Learning.

IEEE transactions on neural networks and learning systems·2026
Same author

From Langmuir films to molecular docking: unraveling the interfacial code of (-)-epigallocatechin gallate binding with milk fat globule membrane components.

Food research international (Ottawa, Ont.)·2026
Same author

Accurately Deciphering Tissue Heterogeneity From Spatial Multi-Modal and Multi-Omics With STransformer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

The effects of virtual reality training on cognitive function in older adults: a systematic review and meta-analysis.

Frontiers in public health·2026
Same journal

Breaking the Stability-Activity-Selectivity Trilemma in Unspecific Peroxygenase through Computation-Based Cross-Regional Combinatorial Mutagenesis.

ACS synthetic biology·2026
Same journal

Sequential Plasmid Curing and Genome Editing in <i>Escherichia coli</i> Nissle 1917.

ACS synthetic biology·2026
Same journal

An Explainable Deep Learning Framework Integrating DNA Sequence and Transcription Initiation Signals for Gene Expression Prediction.

ACS synthetic biology·2026
Same journal

A Multitask Prediction Framework for CircRNAs, Drugs, and Diseases Based on Multi-View Information Integration and Graph Contrastive Learning.

ACS synthetic biology·2026
Same journal

Engineering Modular Cargo Loading Strategies for Carboxysome-Derived Protein Particles.

ACS synthetic biology·2026
Same journal

Suppression of Salmonella Effectors with CRISPRi Controls the Immune Response to Bacterial Therapies.

ACS synthetic biology·2026
查看所有相关文章

相关实验视频

Updated: May 17, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

567

使用重复序列对CRISPR位点进行基于深度学习的分类.

Xingyu Liao1, Yanyan Li1, Yingfu Wu1

  • 1School of Computer Science, Northwestern Polytechnical University, Xi'an, Shanxi 710072, China.

ACS synthetic biology
|April 22, 2025
PubMed
概括
此摘要是机器生成的。

一个新的深度学习工具CRISPRclassify-CNN-Att,仅使用重复序列对CRISPR-Cas系统进行分类,克服了传统cas基因依赖方法对元基因组数据分析的局限性.

关键词:
根据CRISPR的位置分类.这就是CRISPR-Cas系统.深度学习是一种深度学习.重复序列重复序列的时间.自己注意力机制的机制.

更多相关视频

CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

12.4K
Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.5K

相关实验视频

Last Updated: May 17, 2025

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

567
CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

12.4K
Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.5K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • 克里斯普尔-卡斯系统对于基因编辑至关重要,并且越来越多地在元基因组数据中被发现.
  • 传统的分类依赖于识别cas基因,这在元基因组或碎片组合中具有挑战性.
  • 对CRISPR-Cas系统的准确分类对于理解微生物群落和基因组进化至关重要.

研究的目的:

  • 开发一种深度学习方法,仅使用重复序列来对CRISPR-Cas系统进行分类.
  • 为了解决在元基因组数据集中的基因依赖分类的局限性.
  • 为识别孤儿或遥远的CRISPR位点提供一个补充工具.

主要方法:

  • 开发了CRISPRclassify-CNN-Att,这是一种利用卷积神经网络 (CNN) 和自我注意机制的深度学习模型.
  • 采用堆叠策略来处理跨子类型的不平衡样本大小.
  • 应用转移学习以提高对代表性不足的亚型的分类准确性.

主要成果:

  • 在分类多个CRISPR-Cas亚型方面,CRISPRclassify-CNN-Att取得了卓越的表现,特别是那些样本大小较大的亚型.
  • 该方法成功地根据仅基于重复序列的CRISPR位点进行分类,独立于cas基因的存在.
  • 作为传统的基于种类的分类方法的补充方法,已证明有效.

结论:

  • CRISPRclassify-CNN-Att为CRISPR-Cas系统分类提供了一种基于深度学习的新且有效的方法.
  • 这种方法增强了对元基因组数据的分析,使得缺乏基因信息的CRISPR位点能够被分类.
  • 该工具是CRISPR-Cas研究的重大进步,补充了现有的方法.