试验室中调节人类卵细胞成熟:一个基于卵细胞在卵巢组织冷保存期间从小 antral 毛囊中获取的假设
Jesús Cadenas1, Cristina Subiran Adrados2, Ajay Kumar3
1Laboratory of Reproductive Biology, section 5701, University Hospital of Copenhagen, Rigshospitalet, Henrik Harpestrengsvej 6A, 2100, Copenhagen, Denmark. jesus.cadenas.moreno@regionh.dk.
Journal of assisted reproduction and genetics
|April 22, 2025
概括
卵泡刺激激素 (FSH) 和黄素化激素 (LH) 通过改变细胞基因表达和分泌的激素,促进卵细胞成熟,影响人体体体外成熟 (IVM).
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
背景情况:
- 小毛囊 (SAF) 是未成熟卵细胞的来源,用于体外成熟 (IVM).
- 了解IVM期间的荷尔蒙环境对于优化成熟协议至关重要.
研究的目的:
- 在人体IVM过程中表征消耗介质和累积细胞基因表达中的荷尔蒙变化.
- 为了研究FSH和LH在卵细胞成熟中的作用,使用来自冷保存的卵巢组织的SAF.
主要方法:
- 未成熟的卵子经历了42小时的IVM,FSH和LH度各不相同.
- 累积细胞基因表达 (FSHR,LHCGR,AMH,CYP19 A1,INHA) 通过RT-qPCR进行分析.
- 通过ELISA分析了GDF9,AMH,inhibin-B和inhibin-A的消耗媒体.
主要成果:
- 增加FSH下调的FSH受体 (FSHR) 和上调的LH受体 (LHCGR) 表达,与第二阶段 (MII) 过渡相关.
- 在消耗的介质和具有更高FSH的MII卵细胞中,GDF9,AMH和inhibin-B的减少.
- 在MII卵细胞介质中,抑制蛋白A水平较高;FSHR和LHCGR表达与特定激素水平相关.
结论:
- 由FSH诱导的LHCGR表达和FSHR下调是卵细胞成熟的关键.
- 减少GDF9分泌支持LHCGR的表达,而FSH和LH通过像inhibin-A.这样的激素共同促进成熟.
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