孕产妇的年龄与来自euploid胚胎的Blastocoel流体调节介质中的apoptotic基因丰度模式有关:一个试点研究
Dieu Thao Nguyen1, Hannah Archer1, Angel Earle2
1Department of Biomedical Sciences, University of South Carolina School of Medicine Greenville, 607 Grove Rd., Greenville, SC, 29605, USA.
Journal of assisted reproduction and genetics
|April 22, 2025
概括
从ICSI胚胎中调查布拉斯托科尔液体中的基因丰度,发现与母亲年龄相关的差异. 这些发现提供了对老年妇女的亡调节和植入成功的见解.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 胚胎学 胚胎学
背景情况:
- 母亲年龄是影响生育能力和植入成功的重要因素.
- 像ICSI这样的辅助生殖技术被广泛使用,了解胚胎发育至关重要.
研究的目的:
- 在与孕产妇年龄相关的布拉斯托科尔流体条件介质中识别基因和信号通路.
- 为了分析通过ICSI产生的euploid胚胎的全球基因丰富度.
主要方法:
- 布拉斯托科尔液体条件介质的RNA测序 (RNASeq) 来自euploid ICSI产生的胚芽细胞.
- 晚期孕产妇年龄 (≥35) 和年轻患者 (<35) 之间的基因表达的比较.
- 使用定量实时PCR (RT-qPCR) 验证差异表达基因.
主要成果:
- 25个编码蛋白质的基因显示了与母亲年龄相关的差异性丰度.
- 在老年患者中,SHARPIN和BCL2L12基因丰度显著增加.
- 在年轻和年长的母亲年龄组中,在成功植入的病例中,SHARPIN和CASP8的丰度模式不同.
结论:
- 布拉斯托科尔液中的差异性亡基因丰度与母亲的年龄有关.
- 这些发现可能会阐明晚产妇年龄的妇女胚胎的细胞亡调节.
- 鉴定的基因模式可能会影响对植入结果的理解.
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