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分子检测巴托内拉种类的分子检测. 患有血类肉瘤的狗的DNA

Cynthia Robveille¹, Ricardo G Maggi¹, Erin Lashnits²

¹Department of Clinical Sciences, Intracellular Pathogens Research Laboratory, North Carolina State University - College of Veterinary Medicine, Raleigh, North Carolina, United States of America.

|April 22, 2025

概括

固定甲嵌入式 (FFPE) 脏样本在患有血肉瘤 (HSA) 的狗中检测巴顿氏菌感染是不可靠的. 新鲜冷的组织提供卓越的DNA放大精确的巴顿氏菌 spp. 检测. 检测. 在检测.

科学领域:

兽医病理学兽医病理学
传染性疾病传染性疾病
分子诊断学分子诊断学

背景情况:

调查载体传播病原体的作用,如巴顿氏菌 spp. 在犬类瘤中,特别是脏血管肉瘤 (HSA).
之前的研究表明,在美国狗中,巴托内拉细菌病和HSA之间存在潜在的联系.

研究的目的:

确定巴托内拉菌种类的流行率. 美国各地的狗脏HSA中的DNA.
评估组织保存方法 (FFPE与新鲜冷与甲固定) 对巴托内拉DNA放大的影响.

主要方法:

使用定量PCR (qPCR) 和滴滴数字PCR (ddPCR) 来检测巴顿氏菌. 甲固定嵌 (FFPE) 脏组织中的DNA来自患有HSA,非损伤性脏和结节性淋巴细胞增生症的狗.
在FFPE,固定在甲素和来自HSA的狗的新鲜冷组织之间的DNA放大效率的比较.

主要成果:

在患有HSA,非损伤的脏或结节性淋巴细胞增生症的狗的FFPE组织之间,没有发现巴托内拉PCR阳性的显著差异.
巴托内拉SPP. 巴托内拉SPP. 在使用qPCR和ddPCR的新鲜冷组织中,DNA的检测率明显高于使用FFPE组织中的FFPE组织.
滴滴数字PCR (ddPCR) 在与FFPE组织相比,在甲固定组织中显示出更高的放大率.

结论:

由于放大效率低,不建议在狗骨HSA样本中检测巴托内拉DNA.
新鲜冷组织的PCR测试显著增强了巴顿氏菌spp. 检测速率. 检测速率. 检测速率. 检测速率. 检测速率.
对于非新鲜的冷样品,用数字PCR测试的甲固定组织提供了更好的巴顿氏菌DNA检测.

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