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Updated: May 12, 2025

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通过使用液温度来降低冷EM中辐射损伤的影响

Joshua L Dickerson1, Katerina Naydenova1, Mathew J Peet1

  • 1Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|April 22, 2025
PubMed
概括

研究人员通过使用液体温度和纳米电子束来克服电子冷显微镜中的辐射损伤极限. 这一进步使生物分子的更高分辨率的原子结构确定成为可能.

关键词:
低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.电子显微镜的电子显微镜辐射损伤 辐射损伤结构生物学结构生物学水相转换过程中的水相转换.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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